Cloned (Comment) | Organism |
---|---|
gene ADC, real-time RT-PCR enzyme expression analysis | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ethylmercury | 54% inhibition at 0.005 mM | Rattus norvegicus | |
Hg2+ | 85% inhibition at 0.005 mM | Rattus norvegicus | |
methylmercury | 33% inhibition at 0.005 mM | Rattus norvegicus | |
additional information | mercury compounds inhibits the enzyme activity but induce the enzyme expression in PC12 cells, molecular mechanism of mercury compounds' neurotoxicity and enzyme inhibition mechanism, molecular dynamics simulation, product-competitive displacement method, method optimization, overview | Rattus norvegicus | |
phenylmercury | 72% inhibition at 0.005 mM | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | Rattus norvegicus | - |
agmatine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
neuron | - |
Rattus norvegicus | - |
PC-12 cell | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | - |
Rattus norvegicus | agmatine + CO2 | - |
? | |
additional information | enzyme activity assay using cucurbit[7]uril (CB7) and fluorescent dye acridine orange, overview | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADC | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | PLP, molecular dynamics simulation of PLP binding to ADC, overview | Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00000796 | - |
pH 6.0, 37°C | Rattus norvegicus | methylmercury |
Organism | Comment | Expression |
---|---|---|
Rattus norvegicus | mercury compounds inhibits the enzyme activity but induce the enzyme expression in PC12 cells. The results indicate that ADC inhibition in PC12 cells by the four Hg compounds is due to the loss of enzyme activity, not protein level | additional information |
Rattus norvegicus | 0.005 mlM MeHg, EtHg, PhHg and HgCl2 increase the protein expression by 1.23, 1.06, 1.45, and 1.33fold, respectively | up |
General Information | Comment | Organism |
---|---|---|
physiological function | arginine decarboxylase (ADC) is a key enzyme in the central agmatinergic system | Rattus norvegicus |