Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
L89R | the mutant elutes at the expected position for an LdcI dimer (about 150000 Da), the mutant shows about 5fold lower level of activity than wild type and this activity is not inhibited by ppGpp | Escherichia coli |
R206S | the ppGpp-binding site mutant shows wild type oligomerisation profile, the mutant is insensitive to the addition of ppGpp and has activity comparable to wild type LdcI in the absence of ppGpp | Escherichia coli |
R97A | the ppGpp-binding site mutant shows wild type oligomerisation profile, the mutant is insensitive to the addition of ppGpp and has activity comparable to wild type LdcI in the absence of ppGpp | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP | - |
Escherichia coli | |
GTP | - |
Escherichia coli | |
additional information | at pH values lower than 5.0, there is no effect of ppGpp, pppGpp, GDP and GTP on LdcI activity | Escherichia coli | |
ppGpp | addition of ppGpp at low salt concentrations (25-135 mM NaCl depending on the buffer) results in a dramatic inhibition of LdcI activity of about 10fold at pH values higher than 5.0 | Escherichia coli | |
pppGpp | inhibits LdcI only at pH values higher than 6.5 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
L-lysine | at pH 6.5, between 4°C and 10°C | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Escherichia coli | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A9H3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine | - |
Escherichia coli | cadaverine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | the protein is an oligomer of five dimers that associate to form a decamer, X-ray crystallography | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
inducible lysine decarboxylase | - |
Escherichia coli |
LdcI/CadA | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30 | - |
L-lysine | at pH 6.5, between 4°C and 10°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002377 | - |
ppGpp | uncompetitive inhibition, at pH 6.5, between 4°C and 10°C | Escherichia coli | |
0.000374 | - |
ppGpp | noncompetitive inhibition, at pH 6.5, between 4°C and 10°C | Escherichia coli | |
0.002791 | - |
ppGpp | mixed type inhibition, at pH 6.5, between 4°C and 10°C | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | inducible lysine decarboxylase, LdcI/CadA, together with the inner-membrane lysine-cadaverine antiporter, CadB, provide cells with protection against mild acidic conditions (about pH 5.0) | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
71 | - |
L-lysine | at pH 6.5, between 4°C and 10°C | Escherichia coli |