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Literature summary for 4.1.1.18 extracted from

  • Alexopoulos, E.; Kanjee, U.; Snider, J.; Houry, W.A.; Pai, E.F.
    Crystallization and preliminary X-ray analysis of the inducible lysine decarboxylase from Escherichia coli (2008), Acta Crystallogr. Sect. F, 64, 700-706.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, with 18-28% (w/v) PEG 1000, 100 mM NaCl, 100 mM Tris-HCl pH 8.5, 15% (v/v) glycerol, 5 mM tris(2-carboxyethyl)phospine hydrochloride Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A9H3
-
-

Purification (Commentary)

Purification (Comment) Organism
Mono Q column chromatography and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Lys
-
Escherichia coli Cadaverine + CO2
-
?

Subunits

Subunits Comment Organism
decamer x-ray crystallography Escherichia coli

Synonyms

Synonyms Comment Organism
inducible lysine decarboxylase
-
Escherichia coli
LdcI
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 0.1 mM Escherichia coli