Cloned (Comment) | Organism |
---|---|
- |
Selenomonas ruminantium |
Protein Variants | Comment | Organism |
---|---|---|
A44V/G45T/V46P | the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 2.0, compared to 0.83 for the wild-type enzyme | Selenomonas ruminantium |
A44V/G45T/V46P/P54D | the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 3.8, compared to 0.83 for the wild-type enzyme | Selenomonas ruminantium |
A44V/G45T/V46P/P54D/S322A | the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 58, compared to 0.83 for the wild-type enzyme | Selenomonas ruminantium |
A44V/G45T/V46P/P54D/S322T/I326L | the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 13, compared to 0.83 for the wild-type enzyme | Selenomonas ruminantium |
A52C | the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 1.0, compared to 0.83 for the wild-type enzyme | Selenomonas ruminantium |
A52C/P54D | the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 1.6, compared to 0.83 for the wild-type enzyme | Selenomonas ruminantium |
G319W | the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 3.9, compared to 0.83 for the wild-type enzyme | Selenomonas ruminantium |
M50V/A52C/P54D/T55S | the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 1.5, compared to 0.83 for the wild-type enzyme | Selenomonas ruminantium |
P54D | the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 2.2, compared to 0.83 for the wild-type enzyme | Selenomonas ruminantium |
S322A | the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 24, compared to 0.83 for the wild-type enzyme | Selenomonas ruminantium |
S322T/I326L | the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 13, compared to 0.83 for the wild-type enzyme | Selenomonas ruminantium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.96 | - |
L-Orn | pH 6.0, 30°C, wild type enzyme | Selenomonas ruminantium | |
0.98 | - |
L-Orn | pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P | Selenomonas ruminantium | |
1.2 | - |
L-Orn | pH 6.0, 30°C, mutant enzyme S322A | Selenomonas ruminantium | |
1.3 | - |
L-Orn | pH 6.0, 30°C, mutant enzyme A52C | Selenomonas ruminantium | |
1.3 | - |
L-Orn | pH 6.0, 30°C, mutant enzyme G319W | Selenomonas ruminantium | |
1.5 | - |
L-Orn | pH 6.0, 30°C, mutant enzyme S322T/I326L | Selenomonas ruminantium | |
1.5 | - |
L-Lys | pH 6.0, 30°C, wild-type enzyme | Selenomonas ruminantium | |
1.6 | - |
L-Orn | pH 6.0, 30°C, mutant enzyme A52C/P54D | Selenomonas ruminantium | |
1.9 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme A52C | Selenomonas ruminantium | |
2.1 | - |
L-Orn | pH 6.0, 30°C, mutant enzyme P54D | Selenomonas ruminantium | |
2.4 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme A52C/P54D | Selenomonas ruminantium | |
3 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P | Selenomonas ruminantium | |
3.1 | - |
L-Orn | pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D | Selenomonas ruminantium | |
3.3 | - |
L-Orn | pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D/S322T/I326L | Selenomonas ruminantium | |
3.6 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme M50V/A52C/P54D/T55S | Selenomonas ruminantium | |
4 | - |
L-Orn | pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D/S322A | Selenomonas ruminantium | |
4 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme G319W | Selenomonas ruminantium | |
4.5 | - |
L-Orn | pH 6.0, 30°C, mutant enzyme M50V/A52C/P54D/T55S | Selenomonas ruminantium | |
5.7 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme P54D | Selenomonas ruminantium | |
6.7 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme S322A | Selenomonas ruminantium | |
8 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D | Selenomonas ruminantium | |
17 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme S322T/I326L | Selenomonas ruminantium | |
22 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D/S322T/I326L | Selenomonas ruminantium | |
270 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D/S322A | Selenomonas ruminantium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Selenomonas ruminantium | O50657 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Selenomonas ruminantium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Lys | the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 0.83 for the wild-type enzyme | Selenomonas ruminantium | Cadaverine + CO2 | - |
? | |
L-Orn | - |
Selenomonas ruminantium | Putrescine + CO2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme M50V/A52C/P54D/T55S | Selenomonas ruminantium | |
3 | 6 | L-Orn | pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D/S322T/I326L | Selenomonas ruminantium | |
4.7 | - |
L-Orn | pH 6.0, 30°C, mutant enzyme M50V/A52C/P54D/T55S | Selenomonas ruminantium | |
4.8 | - |
L-Orn | pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D/S322A | Selenomonas ruminantium | |
4.8 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme S322A | Selenomonas ruminantium | |
5.5 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D/S322A | Selenomonas ruminantium | |
5.6 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme G319W | Selenomonas ruminantium | |
6.8 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme A52C/P54D | Selenomonas ruminantium | |
7 | - |
L-Orn | pH 6.0, 30°C, mutant enzyme A52C | Selenomonas ruminantium | |
7.2 | - |
L-Orn | pH 6.0, 30°C, mutant enzyme A52C/P54D | Selenomonas ruminantium | |
7.2 | - |
L-Orn | pH 6.0, 30°C, mutant enzyme G319W | Selenomonas ruminantium | |
8.4 | - |
L-Orn | pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P | Selenomonas ruminantium | |
8.6 | - |
L-Orn | pH 6.0, 30°C, wild type enzyme | Selenomonas ruminantium | |
9.8 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D | Selenomonas ruminantium | |
10 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme A52C | Selenomonas ruminantium | |
13 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P | Selenomonas ruminantium | |
13 | - |
L-Orn | pH 6.0, 30°C, mutant enzyme P54D | Selenomonas ruminantium | |
14 | - |
L-Orn | pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D | Selenomonas ruminantium | |
16 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme P54D | Selenomonas ruminantium | |
16 | - |
L-Lys | pH 6.0, 30°C, wild-type enzyme | Selenomonas ruminantium | |
19 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D/S322T/I326L | Selenomonas ruminantium | |
20 | - |
L-Orn | pH 6.0, 30°C, mutant enzyme S322A | Selenomonas ruminantium | |
21 | - |
L-Lys | pH 6.0, 30°C, mutant enzyme S322T/I326L | Selenomonas ruminantium | |
24 | - |
L-Orn | pH 6.0, 30°C, mutant enzyme S322T/I326L | Selenomonas ruminantium |