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Literature summary for 4.1.1.18 extracted from

  • Takatsuka, Y.; Yamaguchi, Y.; Ono, M.; Kamio, Y.
    Gene cloning and molecular characterization of lysine decarboxylase from Selenomonas ruminantium delineate its evolutionary relationship to ornithine decarboxylases from eukaryotes (2000), J. Bacteriol., 182, 6732-6741.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Selenomonas ruminantium

Protein Variants

Protein Variants Comment Organism
A44V/G45T/V46P the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 2.0, compared to 0.83 for the wild-type enzyme Selenomonas ruminantium
A44V/G45T/V46P/P54D the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 3.8, compared to 0.83 for the wild-type enzyme Selenomonas ruminantium
A44V/G45T/V46P/P54D/S322A the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 58, compared to 0.83 for the wild-type enzyme Selenomonas ruminantium
A44V/G45T/V46P/P54D/S322T/I326L the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 13, compared to 0.83 for the wild-type enzyme Selenomonas ruminantium
A52C the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 1.0, compared to 0.83 for the wild-type enzyme Selenomonas ruminantium
A52C/P54D the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 1.6, compared to 0.83 for the wild-type enzyme Selenomonas ruminantium
G319W the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 3.9, compared to 0.83 for the wild-type enzyme Selenomonas ruminantium
M50V/A52C/P54D/T55S the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 1.5, compared to 0.83 for the wild-type enzyme Selenomonas ruminantium
P54D the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 2.2, compared to 0.83 for the wild-type enzyme Selenomonas ruminantium
S322A the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 24, compared to 0.83 for the wild-type enzyme Selenomonas ruminantium
S322T/I326L the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 13, compared to 0.83 for the wild-type enzyme Selenomonas ruminantium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.96
-
L-Orn pH 6.0, 30°C, wild type enzyme Selenomonas ruminantium
0.98
-
L-Orn pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P Selenomonas ruminantium
1.2
-
L-Orn pH 6.0, 30°C, mutant enzyme S322A Selenomonas ruminantium
1.3
-
L-Orn pH 6.0, 30°C, mutant enzyme A52C Selenomonas ruminantium
1.3
-
L-Orn pH 6.0, 30°C, mutant enzyme G319W Selenomonas ruminantium
1.5
-
L-Orn pH 6.0, 30°C, mutant enzyme S322T/I326L Selenomonas ruminantium
1.5
-
L-Lys pH 6.0, 30°C, wild-type enzyme Selenomonas ruminantium
1.6
-
L-Orn pH 6.0, 30°C, mutant enzyme A52C/P54D Selenomonas ruminantium
1.9
-
L-Lys pH 6.0, 30°C, mutant enzyme A52C Selenomonas ruminantium
2.1
-
L-Orn pH 6.0, 30°C, mutant enzyme P54D Selenomonas ruminantium
2.4
-
L-Lys pH 6.0, 30°C, mutant enzyme A52C/P54D Selenomonas ruminantium
3
-
L-Lys pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P Selenomonas ruminantium
3.1
-
L-Orn pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D Selenomonas ruminantium
3.3
-
L-Orn pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D/S322T/I326L Selenomonas ruminantium
3.6
-
L-Lys pH 6.0, 30°C, mutant enzyme M50V/A52C/P54D/T55S Selenomonas ruminantium
4
-
L-Orn pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D/S322A Selenomonas ruminantium
4
-
L-Lys pH 6.0, 30°C, mutant enzyme G319W Selenomonas ruminantium
4.5
-
L-Orn pH 6.0, 30°C, mutant enzyme M50V/A52C/P54D/T55S Selenomonas ruminantium
5.7
-
L-Lys pH 6.0, 30°C, mutant enzyme P54D Selenomonas ruminantium
6.7
-
L-Lys pH 6.0, 30°C, mutant enzyme S322A Selenomonas ruminantium
8
-
L-Lys pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D Selenomonas ruminantium
17
-
L-Lys pH 6.0, 30°C, mutant enzyme S322T/I326L Selenomonas ruminantium
22
-
L-Lys pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D/S322T/I326L Selenomonas ruminantium
270
-
L-Lys pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D/S322A Selenomonas ruminantium

Organism

Organism UniProt Comment Textmining
Selenomonas ruminantium O50657
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Selenomonas ruminantium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Lys the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 0.83 for the wild-type enzyme Selenomonas ruminantium Cadaverine + CO2
-
?
L-Orn
-
Selenomonas ruminantium Putrescine + CO2
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6
-
L-Lys pH 6.0, 30°C, mutant enzyme M50V/A52C/P54D/T55S Selenomonas ruminantium
3 6 L-Orn pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D/S322T/I326L Selenomonas ruminantium
4.7
-
L-Orn pH 6.0, 30°C, mutant enzyme M50V/A52C/P54D/T55S Selenomonas ruminantium
4.8
-
L-Orn pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D/S322A Selenomonas ruminantium
4.8
-
L-Lys pH 6.0, 30°C, mutant enzyme S322A Selenomonas ruminantium
5.5
-
L-Lys pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D/S322A Selenomonas ruminantium
5.6
-
L-Lys pH 6.0, 30°C, mutant enzyme G319W Selenomonas ruminantium
6.8
-
L-Lys pH 6.0, 30°C, mutant enzyme A52C/P54D Selenomonas ruminantium
7
-
L-Orn pH 6.0, 30°C, mutant enzyme A52C Selenomonas ruminantium
7.2
-
L-Orn pH 6.0, 30°C, mutant enzyme A52C/P54D Selenomonas ruminantium
7.2
-
L-Orn pH 6.0, 30°C, mutant enzyme G319W Selenomonas ruminantium
8.4
-
L-Orn pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P Selenomonas ruminantium
8.6
-
L-Orn pH 6.0, 30°C, wild type enzyme Selenomonas ruminantium
9.8
-
L-Lys pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D Selenomonas ruminantium
10
-
L-Lys pH 6.0, 30°C, mutant enzyme A52C Selenomonas ruminantium
13
-
L-Lys pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P Selenomonas ruminantium
13
-
L-Orn pH 6.0, 30°C, mutant enzyme P54D Selenomonas ruminantium
14
-
L-Orn pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D Selenomonas ruminantium
16
-
L-Lys pH 6.0, 30°C, mutant enzyme P54D Selenomonas ruminantium
16
-
L-Lys pH 6.0, 30°C, wild-type enzyme Selenomonas ruminantium
19
-
L-Lys pH 6.0, 30°C, mutant enzyme A44V/G45T/V46P/P54D/S322T/I326L Selenomonas ruminantium
20
-
L-Orn pH 6.0, 30°C, mutant enzyme S322A Selenomonas ruminantium
21
-
L-Lys pH 6.0, 30°C, mutant enzyme S322T/I326L Selenomonas ruminantium
24
-
L-Orn pH 6.0, 30°C, mutant enzyme S322T/I326L Selenomonas ruminantium