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Literature summary for 4.1.1.17 extracted from

  • Choi, H.; Kyeong, H.; Choi, J.; Kim, H.
    Rational design of ornithine decarboxylase with high catalytic activity for the production of putrescine (2014), Appl. Microbiol. Biotechnol., 98, 7483-7490.
    View publication on PubMed
Search for more literature for 4.1.1.17

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling based on the crystal structure of ODC from Lactobacillus 30a, PDB entry 1ORD. The model reveals an unusually deep and narrow shape of the substrate tunnel. Amino acids at the substrate entry site are V156, D160, I163, E165, E689, and Q691 Escherichia coli

Protein Variants

Protein Variants Comment Organism
E165A 1.9fold increase in catalytic efficiency Escherichia coli
E165G 1.8fold increase in catalytic efficiency Escherichia coli
E165S 5.1fold increase in catalytic efficiency Escherichia coli
E165T 36fold increase in catalytic efficiency Escherichia coli
E165V 7.4fold increase in catalytic efficiency Escherichia coli
I163A 2.4fold increase in catalytic efficiency Escherichia coli
I163G 2.4fold increase in catalytic efficiency Escherichia coli
I163S 4.7fold increase in catalytic efficiency Escherichia coli
I163T 17.6fold increase in catalytic efficiency Escherichia coli
I163T/E165T 62.5fold increase in catalytic efficiency Escherichia coli
I163V 3.4fold increase in catalytic efficiency Escherichia coli
I163V/E165V 22.7fold increase in catalytic efficiency Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 L-ornithine mutant I163T, pH 7.0, temperature not specified in the publication Escherichia coli
0.6
-
 L-ornithine mutant I163T/E165T, pH 7.0, temperature not specified in the publication Escherichia coli
0.7
-
 L-ornithine mutant E165T, pH 7.0, temperature not specified in the publication Escherichia coli
1.1
-
 L-ornithine mutant I163V/E165V, pH 7.0, temperature not specified in the publication Escherichia coli
1.3
-
 L-ornithine mutant I163V, pH 7.0, temperature not specified in the publication Escherichia coli
1.4
-
 L-ornithine mutant E165V, pH 7.0, temperature not specified in the publication Escherichia coli
1.5
-
 L-ornithine mutant I163A, pH 7.0, temperature not specified in the publication Escherichia coli
1.5
-
 L-ornithine mutant I163S, pH 7.0, temperature not specified in the publication Escherichia coli
1.7
-
 L-ornithine mutant I163G, pH 7.0, temperature not specified in the publication Escherichia coli
1.9
-
 L-ornithine mutant E165S, pH 7.0, temperature not specified in the publication Escherichia coli
2.4
-
 L-ornithine mutant E165A, pH 7.0, temperature not specified in the publication Escherichia coli
3
-
 L-ornithine mutant E165G, pH 7.0, temperature not specified in the publication Escherichia coli
3.3
-
 L-ornithine wild-type, pH 7.0, temperature not specified in the publication Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P21169
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine
-
 Escherichia coli putrescine + CO2
-
 ?

Synonyms

Synonyms Comment Organism
SpeC
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4
-
 L-ornithine wild-type, pH 7.0, temperature not specified in the publication Escherichia coli
3.6
-
 L-ornithine mutant I163A, pH 7.0, temperature not specified in the publication Escherichia coli
4.2
-
 L-ornithine mutant I163G, pH 7.0, temperature not specified in the publication Escherichia coli
4.4
-
 L-ornithine mutant I163V, pH 7.0, temperature not specified in the publication Escherichia coli
4.8
-
 L-ornithine mutant E165A, pH 7.0, temperature not specified in the publication Escherichia coli
5.6
-
 L-ornithine mutant E165G, pH 7.0, temperature not specified in the publication Escherichia coli
7.4
-
 L-ornithine mutant I163S, pH 7.0, temperature not specified in the publication Escherichia coli
10.1
-
 L-ornithine mutant E165S, pH 7.0, temperature not specified in the publication Escherichia coli
10.1
-
 L-ornithine mutant I163T, pH 7.0, temperature not specified in the publication Escherichia coli
10.9
-
 L-ornithine mutant E165V, pH 7.0, temperature not specified in the publication Escherichia coli
24.8
-
 L-ornithine mutant E165T, pH 7.0, temperature not specified in the publication Escherichia coli
25.7
-
 L-ornithine mutant I163V/E165V, pH 7.0, temperature not specified in the publication Escherichia coli
38.5
-
 L-ornithine mutant I163T/E165T, pH 7.0, temperature not specified in the publication Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1
-
 L-ornithine wild-type, pH 7.0, temperature not specified in the publication Escherichia coli
1.8
-
 L-ornithine mutant I163T, pH 7.0, temperature not specified in the publication Escherichia coli
1.9
-
 L-ornithine mutant E165G, pH 7.0, temperature not specified in the publication Escherichia coli
2
-
 L-ornithine mutant E165A, pH 7.0, temperature not specified in the publication Escherichia coli
2.3
-
 L-ornithine mutant I163V/E165V, pH 7.0, temperature not specified in the publication Escherichia coli
2.4
-
 L-ornithine mutant I163A, pH 7.0, temperature not specified in the publication Escherichia coli
2.5
-
 L-ornithine mutant I163G, pH 7.0, temperature not specified in the publication Escherichia coli
3.5
-
 L-ornithine mutant I163V, pH 7.0, temperature not specified in the publication Escherichia coli
3.7
-
 L-ornithine mutant E165T, pH 7.0, temperature not specified in the publication Escherichia coli
4.8
-
 L-ornithine mutant I163S, pH 7.0, temperature not specified in the publication Escherichia coli
5.2
-
 L-ornithine mutant E165S, pH 7.0, temperature not specified in the publication Escherichia coli
6.4
-
 L-ornithine mutant I163T/E165T, pH 7.0, temperature not specified in the publication Escherichia coli
7.6
-
 L-ornithine mutant E165V, pH 7.0, temperature not specified in the publication Escherichia coli