Cloned (Comment) | Organism |
---|---|
expression of wild-type and chimeric mutant enzymes in COS cells | Crithidia fasciculata |
expression of wild-type and chimeric mutant enzymes in COS cells | Leishmania donovani |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of chimeric mutant enzymes consisting of sequence from the Crithidia fasciculata and the Leishmania donovani enzymes, e.g. exchanging the C-terminal PEST region, which show altered protein stability compared to the wild-type enzymes, overview | Crithidia fasciculata |
additional information | construction of chimeric mutant enzymes consisting of sequence from the Crithidia fasciculata and the Leishmania donovani enzymes, e.g. exchanging the C-terminal PEST region, which show altered protein stability compared to the wild-type enzymes, overview | Leishmania donovani |
General Stability | Organism |
---|---|
Crithidia fasciculata ODC, in contrast to other phylogentically related enzymes, is rapidly degraded also in mammalian systems, and it contains several sequence elements essential for the rapid turnover of the protein, these regions are mainly located in the central part of the enzyme, overview, half-life of the wild-type enzyme is 3.2 h | Crithidia fasciculata |
Leishmania donovani ODC has a very fast turnover in mammalian cells, but is a stable enzyme, half-life of the wild-type enzyme is 5.5 h | Leishmania donovani |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Crithidia fasciculata | Crithidia fasciculata ODC, in contrast to other phylogentically related enzymes, is rapidly degraded also in mammalian systems, and it contains several sequence elements essential for the rapid turnover of the protein, these regions are mainly located in the central part of the enzyme, overview | ? | - |
? | |
additional information | Leishmania donovani | Leishmania donovani ODC has a very fast turnover in mammalian cells, but is a stable enzyme | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crithidia fasciculata | P90517 | - |
- |
Leishmania donovani | P27116 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Crithidia fasciculata ODC, in contrast to other phylogentically related enzymes, is rapidly degraded also in mammalian systems, and it contains several sequence elements essential for the rapid turnover of the protein, these regions are mainly located in the central part of the enzyme, overview | Crithidia fasciculata | ? | - |
? | |
additional information | Leishmania donovani ODC has a very fast turnover in mammalian cells, but is a stable enzyme | Leishmania donovani | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ODC | - |
Crithidia fasciculata |
ODC | - |
Leishmania donovani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Crithidia fasciculata |
7.5 | - |
assay at | Leishmania donovani |