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Literature summary for 4.1.1.17 extracted from
- Characterization of ornithine decarboxylase from Pseudomonas syringae pv. phaseolicola and its inhibition by phaseolotoxin (2004), Physiol. Mol. Plant Pathol., 63, 57-63.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| enzyme is sensitive to freeze-thawing | Pseudomonas savastanoi pv. phaseolicola |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-difluoromethylornithine | - |
Pseudomonas savastanoi pv. phaseolicola |  |
| Phaseolotoxin | - |
Pseudomonas savastanoi pv. phaseolicola | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
L-ornithine | pH 7.3, 37°C | Pseudomonas savastanoi pv. phaseolicola | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
4 * 41000, SDS-PAGE, or 3 * 41000 + 1 * 56000, SDS-PAGE | Pseudomonas savastanoi pv. phaseolicola |
| 56000 | - |
4 * 41000, SDS-PAGE, or 3 * 41000 + 1 * 56000, SDS-PAGE | Pseudomonas savastanoi pv. phaseolicola |
| 160000 | - |
gel filtration | Pseudomonas savastanoi pv. phaseolicola |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas savastanoi pv. phaseolicola | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas savastanoi pv. phaseolicola |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.3 | - |
pH 7.3, 37°C | Pseudomonas savastanoi pv. phaseolicola |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, presence of 0.04 mM pyridoxal 5'-phosphate , 1.0 mM dithiothreitol, 10% glycerol, stable for at least 5 months | Pseudomonas savastanoi pv. phaseolicola |
| 4°C, presence of 0.04 mM pyridoxal 5'-phosphate , 1.0 mM dithiothreitol, stable for 2 weeks | Pseudomonas savastanoi pv. phaseolicola |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-ornithine | - |
Pseudomonas savastanoi pv. phaseolicola | putrescine + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 41000, SDS-PAGE, or 3 * 41000 + 1 * 56000, SDS-PAGE | Pseudomonas savastanoi pv. phaseolicola |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
- |
Pseudomonas savastanoi pv. phaseolicola |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
10% of the activity at pH 7.3 | Pseudomonas savastanoi pv. phaseolicola |
| 8.8 | - |
38% of the activity at pH 7.3 | Pseudomonas savastanoi pv. phaseolicola |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Pseudomonas savastanoi pv. phaseolicola | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
Phaseolotoxin | pH 7.3, 37°C | Pseudomonas savastanoi pv. phaseolicola | |
| 0.026 | - |
alpha-difluoromethylornithine | pH 7.3, 37°C | Pseudomonas savastanoi pv. phaseolicola | |
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