Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Nicotiana glutinosa |
Protein Variants | Comment | Organism |
---|---|---|
C338A | 14% of wild-type L-ornithine decarboxylase activity | Nicotiana glutinosa |
C377A | 4% of wild-type L-ornithine decarboxylase activity | Nicotiana glutinosa |
C96A | 42% of wild-type L-ornithine decarboxylase activity | Nicotiana glutinosa |
K95A | 1% of wild-type L-ornithine decarboxylase activity | Nicotiana glutinosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | 0.001 mM, approx. 80% inhibition of ODC activity after 8 min | Nicotiana glutinosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
L-ornithine | - |
Nicotiana glutinosa | |
1.59 | - |
L-lysine | - |
Nicotiana glutinosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46500 | - |
2 * 46500, SDS-PAGE | Nicotiana glutinosa |
92000 | - |
native PAGE | Nicotiana glutinosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine | Nicotiana glutinosa | - |
1,5-diaminopentane + CO2 | - |
? | |
L-ornithine | Nicotiana glutinosa | - |
putrescine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana glutinosa | Q9FPK5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant ODC | Nicotiana glutinosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine | - |
Nicotiana glutinosa | 1,5-diaminopentane + CO2 | - |
? | |
L-ornithine | - |
Nicotiana glutinosa | putrescine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 46500, SDS-PAGE | Nicotiana glutinosa |
Synonyms | Comment | Organism |
---|---|---|
ODC | - |
Nicotiana glutinosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
half-life 70 min | Nicotiana glutinosa |
50 | - |
half-life 20 min | Nicotiana glutinosa |
60 | - |
half-life 5 min | Nicotiana glutinosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
L-lysine | - |
Nicotiana glutinosa | |
77.8 | - |
L-ornithine | - |
Nicotiana glutinosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
L-lysine decarboxylation | Nicotiana glutinosa |
8 | - |
L-ornithine decarboxylation | Nicotiana glutinosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | L-lysine decarboxylation, approx. 40% of maximal activity at pH 7.5 | Nicotiana glutinosa |
7.2 | 8.7 | L-ornithine decarboxylation, approx. 35% of maximal activity at pH 7.2 | Nicotiana glutinosa |