Cloned (Comment) | Organism |
---|---|
expression of ODC domain, i.e. rPfODC, and ODC domain in conjunction with the preceeding part of the hinge region of the bifunctional ODC/AdoMetDC, i.e.rPfHinge-ODC, in Escherichia coli | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-difluoroornithine | - |
Plasmodium falciparum | |
CGP52622A | 3-amino-oxy-1-propanamine analog, 0.00006 mM, 50% inhibition of recombinant Pf-Hinge-ODC | Plasmodium falciparum | |
CGP54619A | 3-amino-oxy-1-propanamine analog, 0.000025 mM, 50% inhibition of recombinant Pf-Hinge-ODC | Plasmodium falciparum | |
putrescine | - |
Plasmodium falciparum | |
spermidine | 1 mM, 61% inhibition of recombinant-Hinge-ODC and 63% inhibition of recombinant PfODC | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
L-ornithine | recombinant Pf-Hinge-ODC | Plasmodium falciparum | |
0.16 | - |
L-ornithine | recombinant PfODC domain | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine | Plasmodium falciparum | - |
putrescine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant PfODC and Pf-Hinge-ODC | Plasmodium falciparum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0043 | - |
recombinant pF-Hinge-ODC | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine | - |
Plasmodium falciparum | putrescine + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ODC | - |
Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
L-ornithine | recombinant PfODC domain | Plasmodium falciparum | |
0.006 | - |
L-ornithine | recombinant Pf-Hinge-ODC | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | recombinant PfODC domain | Plasmodium falciparum |
7.5 | 8.5 | recombinant Pf-Hinge-ODC | Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000008 | - |
CGP54619A | - |
Plasmodium falciparum | |
0.00002 | - |
CGP52622A | - |
Plasmodium falciparum | |
0.05 | - |
putrescine | recombinant Pf-Hinge-ODC | Plasmodium falciparum | |
0.09 | - |
alpha-difluoroornithine | recombinant Pf-Hinge-ODC | Plasmodium falciparum | |
0.16 | - |
putrescine | recombinant PfODC domain | Plasmodium falciparum |