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Literature summary for 4.1.1.17 extracted from

  • Vitali, J.; Carroll, D.; Chaudhry, R.G.; Hackert, M.L.
    Three-dimensional structure of the Gly121Tyr dimeric form of ornithine decarboxylase from Lactobacillus 30a (1999), Acta Crystallogr. Sect. D, 55, 1978-1985.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dGTP reactivation of ODC at pH values above 7.0 Lactobacillus sp. 30a
GDP reactivation of ODC at pH values above 7.0 Lactobacillus sp. 30a
GTP reactivation of ODC at pH values above 7.0, dodecameric ODC binds 2 GTPs per dimer at pH 5.8 Lactobacillus sp. 30a

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Lactobacillus sp. 30a

Crystallization (Commentary)

Crystallization (Comment) Organism
G121Y mutant dimer at pH 7.0 in the presence of GTP, purified ODC is dialyzed against 100 mM sodium-HCl, pH 6.5, 1 mM dithiothreitol, 0.2 mM pyridoxal 5'-phosphate, 0.02% sodium azide and 0.5 mM EDTA and concentrated to 20 mg/ml, crystals are grown at room temperature by the sitting-drop method from reservoirs containing 30% polyethylene glycol 3350, 200 mM ammonium acetate and 100 mM Na-HEPES, pH 7.0, crystals diffract to 2.7 A resolution Lactobacillus sp. 30a

Protein Variants

Protein Variants Comment Organism
G121Y Gly121 is buried in the dimer-dimer interface, mutation of Gly121 into Tyr prevents association of dimers into dodecamers, G121Y shows similar activity as native ODC Lactobacillus sp. 30a

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
12 * 83000, SDS-PAGE, dodecamer composed of six homodimers Lactobacillus sp. 30a
990600
-
-
Lactobacillus sp. 30a

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-ornithine Lactobacillus sp. 30a
-
putrescine + CO2
-
?

Organism

Organism UniProt Comment Textmining
Lactobacillus sp. 30a
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant ODC Lactobacillus sp. 30a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine
-
Lactobacillus sp. 30a putrescine + CO2
-
?

Subunits

Subunits Comment Organism
dodecamer 12 * 83000, SDS-PAGE, dodecamer composed of six homodimers Lactobacillus sp. 30a

Synonyms

Synonyms Comment Organism
ODC
-
Lactobacillus sp. 30a

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
-
Lactobacillus sp. 30a

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Lactobacillus sp. 30a