Literature summary for 4.1.1.15 extracted from

Shi, F.; Xie, Y.; Jiang, J.; Wang, N.; Li, Y.; Wang, X.
Directed evolution and mutagenesis of glutamate decarboxylase from Lactobacillus brevis Lb85 to broaden the range of its activity toward a near-neutral pH (2014), Enzyme Microb. Technol., 61-62, 35-43.

Search for more literature for 4.1.1.15

Cloned(Commentary)

Cloned (Comment)	Organism
gene gadB1, expression of wild-type and mutant enzymes in Corynebacterium glutamicum strain ATCC 13032 leads to increased 4-aminobutanoate levels in the transgenic bacterium	Levilactobacillus brevis

Protein Variants

Protein Variants	Comment	Organism
E312S	site-directed mutagenesis	Levilactobacillus brevis
additional information	directed evolution of GadB1 using site-specific mutagenesis, mutant screening	Levilactobacillus brevis
T17I/D294G/E312S/Q346H	site-directed mutagenesis	Levilactobacillus brevis
T17I/D294G/Q346H	site-directed mutagenesis, the mutant has increased catalytic efficiency, showing 13.1 and 43.2fold of wild-type GadB1 activity at pH 4.6 and pH 6.0, respectively	Levilactobacillus brevis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-glutamate	Levilactobacillus brevis	-	4-aminobutanoate + CO2	-	?
L-glutamate	Levilactobacillus brevis Lb85	-	4-aminobutanoate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Levilactobacillus brevis	-	gene gadB1	-
Levilactobacillus brevis Lb85	-	gene gadB1	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamate	-	Levilactobacillus brevis	4-aminobutanoate + CO2	-	?
L-glutamate	consumption of one H+	Levilactobacillus brevis	4-aminobutanoate + CO2	-	?
L-glutamate	-	Levilactobacillus brevis Lb85	4-aminobutanoate + CO2	-	?
L-glutamate	consumption of one H+	Levilactobacillus brevis Lb85	4-aminobutanoate + CO2	-	?

Synonyms

Synonyms	Comment	Organism
GADB1	-	Levilactobacillus brevis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4	5	-	Levilactobacillus brevis

pH Range

pH Minimum	pH Maximum	Comment	Organism
additional information	-	the enzyme significantly loses activity at near-neutral pH	Levilactobacillus brevis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)