Cloned (Comment) | Organism |
---|---|
gene gad, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli strains BL21 and JM109 | Levilactobacillus brevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, recombinant enzyme, SDS-PAGE | Levilactobacillus brevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | Levilactobacillus brevis | - |
4-aminobutanoate + CO2 | - |
? | |
L-glutamate | Levilactobacillus brevis CGMCC 1306 | - |
4-aminobutanoate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Levilactobacillus brevis | D6PXK5 | gene gad | - |
Levilactobacillus brevis CGMCC 1306 | D6PXK5 | gene gad | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Levilactobacillus brevis | 4-aminobutanoate + CO2 | - |
? | |
L-glutamate | - |
Levilactobacillus brevis CGMCC 1306 | 4-aminobutanoate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, recombinant enzyme, SDS-PAGE | Levilactobacillus brevis |
Synonyms | Comment | Organism |
---|---|---|
GAD | - |
Levilactobacillus brevis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | - |
- |
Levilactobacillus brevis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 65 | activity range, profile overview | Levilactobacillus brevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
- |
Levilactobacillus brevis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | activity range, profile overview | Levilactobacillus brevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | binding of pyridoxal 5'-phosphate as well as to the active site residues Thr215 and Asp246 that promote decarboxylation | Levilactobacillus brevis |
General Information | Comment | Organism |
---|---|---|
additional information | binding of pyridoxal 5'-phosphate as well as to the active site residues Thr215 and Asp246 that promote decarboxylation | Levilactobacillus brevis |