Literature summary for 4.1.1.12 extracted from

Lima, S.; Sundararaju, B.; Huang, C.; Khristoforov, R.; Momany, C.; Phillips, R.S.
The crystal structure of the Pseudomonas dacunhae aspartate-beta-decarboxylase dodecamer reveals an unknown oligomeric assembly for a pyridoxal-5-phosphate-dependent enzyme (2009), J. Mol. Biol., 388, 98-108.

Search for more literature for 4.1.1.12

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Pseudomonas dacunhae

Crystallization (Commentary)

Crystallization (Comment)	Organism
microbatch-under-oil method, using 100 mM MgCl2, 100 mM Tris, pH 8.5, 25% (v/v) polyethylene glycol (PEG) 400, and 10% (v/v) glycerol	Pseudomonas dacunhae

Protein Variants

Protein Variants	Comment	Organism
R37A	the mutant shows reduced activity	Pseudomonas dacunhae
S298R	the mutant shows very low activity	Pseudomonas dacunhae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.4	-	L-Asp	wild type enzyme, in 50 mM potassium phosphate, at pH 7.0 and 37°C	Pseudomonas dacunhae
16.9	-	L-Asp	mutant enzyme R37A, in 50 mM potassium phosphate, at pH 7.0 and 37°C	Pseudomonas dacunhae

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas dacunhae	Q845W8	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation and Sepharose CL-6B resin gel filtration	Pseudomonas dacunhae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Asp	-	Pseudomonas dacunhae	L-Ala + CO2	-	?

Subunits

Subunits	Comment	Organism
homododecamer	x-ray crystallography	Pseudomonas dacunhae

Synonyms

Synonyms	Comment	Organism
ABDC	-	Pseudomonas dacunhae
aspartate 4-carboxylyase	-	Pseudomonas dacunhae
aspartate-beta-decarboxylase	-	Pseudomonas dacunhae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.7	-	L-Asp	mutant enzyme R37A, in 50 mM potassium phosphate, at pH 7.0 and 37°C	Pseudomonas dacunhae
4.3	-	L-Asp	wild type enzyme, in 50 mM potassium phosphate, at pH 7.0 and 37°C	Pseudomonas dacunhae

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Pseudomonas dacunhae

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Release 2023.1 (January 2023)