Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Pseudomonas sp. |
Protein Variants | Comment | Organism |
---|---|---|
P257H | the mutation results in inactivation of the enzyme, but does not affect the overall structure (0.08% of the wild type enzymatic activity) | Pseudomonas sp. |
S298R | the mutation disassembles the dodecameric L-aspartate 4-decarboxylase into inactive dimers (0.1% of the wild type enzymatic activity) | Pseudomonas sp. |
Y207H | the mutation results in inactivation of the enzyme, but does not affect the overall structure (0.08% of the wild type enzymatic activity) | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
strain ATCC 19121 | - |
Purification (Comment) | Organism |
---|---|
HiTrap chelating column chromatography | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.02 | - |
mutant enzyme P257H, in acetate buffer (pH 5.0) at 40°C | Pseudomonas sp. |
0.02 | - |
mutant enzyme S298R, in acetate buffer (pH 5.0) at 40°C | Pseudomonas sp. |
0.02 | - |
mutant enzyme Y207H, in acetate buffer (pH 5.0) at 40°C | Pseudomonas sp. |
20.3 | - |
wild type enzyme, in acetate buffer (pH 5.0) at 40°C | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Asp | - |
Pseudomonas sp. | L-Ala + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | at pH 5.0, X-ray crystallography | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
Asd | - |
Pseudomonas sp. |
L-aspartate 4-decarboxylase | - |
Pseudomonas sp. |