Cloned (Comment) | Organism |
---|---|
genetic mapping, sequence comparisons and phylogenetic analysis, quantitative RT-PCR enzyme expression analysis | Bombyx mori |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a bp mutant strain 16-100 (bp/bp). In the bp mutant, a SINE-like transposon with a length of 493 bp is detected about 2.2 kb upstream of the transcriptional start site of the BmADC gene. This insertion causes a sharp reduction in BmADC transcript levels in bp mutants, leading to deficiency of beta-alanine and N-beta-alanyl dopamine (NBAD), but accumulation of dopamine. The mutant is specifically melanized only at the pupal stage. Following injection of beta-alanine into bp mutants, the color pattern is reverted to that of the wild-type silkworms. Additionally, melanic pupae resulting from knockdown of BmADC in the wild-type strain are obtained by RNAi of BmADC | Bombyx mori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | Bombyx mori | - |
beta-alanine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | H9JRC8 | three wild-type strains, Dazao (+ bp/+ bp), C108 (+ bp/+ bp), and N4 (+ bp/+ bp) | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
larva | - |
Bombyx mori | - |
additional information | temporal expression pattern of BmADC from the wandering to moth stages | Bombyx mori | - |
pupa | - |
Bombyx mori | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | - |
Bombyx mori | beta-alanine + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aspartate decarboxylase | - |
Bombyx mori |
BmADC | - |
Bombyx mori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Bombyx mori |
General Information | Comment | Organism |
---|---|---|
malfunction | a mutation in the aspartate decarboxylase gene (BmADC) causes melanized pupae with melanization specifically only at the pupal stage, the bp mutant phenotype. In the bp mutant, a SINE-like transposon insertion causes a sharp reduction in BmADC transcript levels in bp mutants, leading to deficiency of beta-alanine and N-beta-alanyl dopamine (NBAD), but accumulation of dopamine. Enzyme knockout also leads to the melanic pupae. The color pattern is reverted to that of the wild-type silkworms following injection of beta-alanine into bp mutants. Larvaeal bp phenotype, overview. Absence of beta-alanine and excessive accumulation of dopamine in the bp mutant | Bombyx mori |
metabolism | the enzyme is involved in the melanin metabolism pathway and in the melanin metabolism from dopamine to N-beta-alanyl dopamine (NBAD) | Bombyx mori |
physiological function | enzyme BmADC plays a crucial role in melanin metabolism and in the pigmentation pattern of the silkworm pupal stage | Bombyx mori |