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Literature summary for 4.1.1.103 extracted from

  • Yoshida, M.; Fukuhara, N.; Oikawa, T.
    Thermophilic, reversible gamma-resorcylate decarboxylase from Rhizobium sp. strain MTP-10005: purification, molecular characterization, and expression (2004), J. Bacteriol., 186, 6855-6863.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37500
-
4 * 37500, SDS-PAGE Rhizobium sp.
151000
-
gel filtration Rhizobium sp.

Organism

Organism UniProt Comment Textmining
Rhizobium sp. Q60GU1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhizobium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-dihydroxybenzene + CO2
-
Rhizobium sp. 2,6-dihydroxybenzoate
-
r
2,6-dihydroxybenzoate
-
Rhizobium sp. 1,3-dihydroxybenzene + CO2
-
r
additional information no substrates: 2,4-dihydroxybenzoate, 2,5-dihydroxybenzoate, 3,4-dihydroxybenzoate, 3,5-dihydroxybenzoate, 2-hydroxybenzoate, or 3-hydroxybenzoate Rhizobium sp. ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 37500, SDS-PAGE Rhizobium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
half-life 122 min Rhizobium sp.

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor required Rhizobium sp.

Expression

Organism Comment Expression
Rhizobium sp. the enzyme is induced specifically by the addition of gamma-resorcylate to the medium up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.098
-
1,3-dihydroxybenzene pH 7, 30°C Rhizobium sp.
13.4
-
2,6-dihydroxybenzoate pH 7, 30°C Rhizobium sp.