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Literature summary for 4.1.1.1 extracted from

  • Wu, X.; Fei, M.; Chen, Y.; Wang, Z.; Chen, Y.
    Enzymatic synthesis of L-norephedrine by coupling recombinant pyruvate decarboxylase and omega-transaminase (2014), Appl. Microbiol. Biotechnol., 98, 7399-7408.
    View publication on PubMed
Search for more literature for 4.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in Escherichia coli strain BL21(DE3) Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 x * 62000, recombinant enzyme, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzaldehyde + pyruvate
-
 Saccharomyces cerevisiae L-phenylacetylcarbinol + CO2
-
 ?
additional information enzymatic stereoselective synthesis of L-norephedrine by coupling recombinant R-selective pyruvate decarboxylase from from Saccharomyces cerevisiae and an S-selective omega-transaminase from Vibrio fluvialis JS17, method optimization, overview Saccharomyces cerevisiae ?
-
 ?

Subunits

Subunits Comment Organism
? x * 62000, recombinant enzyme, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
R-selective pyruvate decarboxylase
-
 Saccharomyces cerevisiae
SCPDC
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 synthesis of L-norephedrine in a coupled reaction with omega-transaminase from Vibrio fluvialis JS17 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 synthesis of L-norephedrine in a coupled reaction with omega-transaminase from Vibrio fluvialis JS17 Saccharomyces cerevisiae