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Literature summary for 4.1.1.1 extracted from

  • Yun, H.; Kim, B.
    Enzymatic production of (R)-phenylacetylcarbinol by pyruvate decarboxylase from Zymomonas mobilis (2008), Biotechnol. Bioprocess Eng., 13, 372-376.
No PubMed abstract available
Search for more literature for 4.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 Zymomonas mobilis

Protein Variants

Protein Variants Comment Organism
W329M the carboligase activity of the mutant is 2.8% as high as the decarboxylase activity which is about 10fold higher than the wild type enzyme Zymomonas mobilis

Inhibitors

Inhibitors Comment Organism Structure
benzaldehyde after a 20 h incubation with 30 mM benzaldehyde, the residual activity is 84% of the initial activity, enzyme stability dramatically decreases in the presence of 200 mM benzaldehyde (27% residual activity after 3 h incubation) Zymomonas mobilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Zymomonas mobilis

Organism

Organism UniProt Comment Textmining
Zymomonas mobilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzaldehyde + pyruvate
-
 Zymomonas mobilis (1R)-phenylacetylcarbinol + CO2
-
 ir
pyruvate
-
 Zymomonas mobilis acetaldehyde + CO2
-
 ir

Synonyms

Synonyms Comment Organism
PDC
-
 Zymomonas mobilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 for decarboxylation Zymomonas mobilis
6.5
-
 for carboligation Zymomonas mobilis

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
 Zymomonas mobilis