Literature summary for 3.9.1.3 extracted from

Baeumer, N.; Maeurer, A.; Krieglstein, J.; Klumpp, S.
Expression of protein histidine phosphatase in Escherichia coli, purification, and determination of enzyme activity (2007), Methods Mol. Biol., 365, 247-260.

Search for more literature for 3.9.1.3

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Escherichia coli	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	Mg2+ ions are recommended upon phosphorylation	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
14000	-	x * 14000, SDS-PAGE	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
[ATP-citrate lyase]-N-phospho-L-histidine + H2O	Escherichia coli	-	[ATP-citrate lyase]-L-histidine + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[ATP-citrate lyase]-N-phospho-L-histidine + H2O	-	Escherichia coli	[ATP-citrate lyase]-L-histidine + phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 14000, SDS-PAGE	Escherichia coli

Synonyms

Synonyms	Comment	Organism
PHP	-	Escherichia coli
protein histidine phosphatase	-	Escherichia coli

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Release 2023.1 (January 2023)