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Literature summary for 3.9.1.3 extracted from

  • Ek, P.; Pettersson, G.; Ek, B.; Gong, F.; Li, J.P.; Zetterqvist, O.
    Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase (2002), Eur. J. Biochem., 269, 5016-5023.
    View publication on PubMed
Search for more literature for 3.9.1.3

Inhibitors

Inhibitors Comment Organism Structure
additional information insensitive towards okadaic acid and EDTA Homo sapiens
additional information insensitive towards okadaic acid and EDTA Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Sus scrofa 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13700
-
 x * 13700, mass spectrometry Sus scrofa
13700
-
 x * 13700, mass spectrometry Homo sapiens
13701
-
 x * 13701, calculated from amino acid sequence Sus scrofa
13701
-
 x * 13701, calculated from amino acid sequence Homo sapiens
14000
-
 x * 14000, SDS-PAGE Sus scrofa
14000
-
 x * 14000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a [protein]-N-phospho-L-histidine + H2O Sus scrofa
-
 a [protein]-L-histidine + phosphate
-
 ?
a [protein]-N-phospho-L-histidine + H2O Homo sapiens
-
 a [protein]-L-histidine + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NRX4
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography, Sephadex G-200 gel filtration, Mono-Q column chromatography, and Superose 12 gel filtration Sus scrofa
DEAE-cellulose column chromatography, Sephadex G-200 gel filtration, Mono-Q column chromatography, and Superose 12 gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Homo sapiens
-
liver
-
 Sus scrofa
-
skeletal muscle
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0028
-
 crude enzyme, at pH 7.5 and 30°C Sus scrofa
2.72
-
 after 971fold purification, at pH 7.5 and 30°C Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a [protein]-N-phospho-L-histidine + H2O
-
 Sus scrofa a [protein]-L-histidine + phosphate
-
 ?
a [protein]-N-phospho-L-histidine + H2O
-
 Homo sapiens a [protein]-L-histidine + phosphate
-
 ?
succinyl-L-Ala-N-phospho-L-His-L-Pro-L-Phe 4-nitroanilide + H2O
-
 Sus scrofa succinyl-L-Ala-L-His-L-Pro-L-Phe 4-nitroanilide + phosphate
-
 ?
succinyl-L-Ala-N-phospho-L-His-L-Pro-L-Phe 4-nitroanilide + H2O
-
 Homo sapiens succinyl-L-Ala-L-His-L-Pro-L-Phe 4-nitroanilide + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 14000, SDS-PAGE Sus scrofa
? x * 14000, SDS-PAGE Homo sapiens
? x * 13700, mass spectrometry Sus scrofa
? x * 13700, mass spectrometry Homo sapiens
? x * 13701, calculated from amino acid sequence Sus scrofa
? x * 13701, calculated from amino acid sequence Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Sus scrofa
7.5
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5.6 9 at pH 5.6, the activity is 2% of that at pH 7.5, and no activity is detected at pH 9.0 Sus scrofa
5.6 9 at pH 5.6, the activity is 2% of that at pH 7.5, and no activity is detected at pH 9.0 Homo sapiens