Literature summary for 3.9.1.3 extracted from

Klumpp, S.; Ma, N.T.; Baeumer, N.; Bechmann, G.; Krieglstein, J.
Relevance of glycine and cysteine residues as well as N- and C-terminals for the activity of protein histidine phosphatase (2010), Biochim. Biophys. Acta, 1804, 206-211.

Search for more literature for 3.9.1.3

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
C69A	the mutation does not affect enzyme activity	Homo sapiens
C69A/C71A	the mutations do not affect enzyme activity	Homo sapiens
C69A/C71A/C73A	the mutations do not affect enzyme activity	Homo sapiens
C69A/C73A	the mutation s do not affect enzyme activity	Homo sapiens
C71A	the mutation does not affect enzyme activity	Homo sapiens
C71A/C73A	the mutations do not affect enzyme activity	Homo sapiens
C73A	the mutation does not affect enzyme activity	Homo sapiens
G75A	inacvtive	Homo sapiens
G75A/G77A/S80A	inactive	Homo sapiens
G77A	inacvtive	Homo sapiens
additional information	deletion of 9 N-terminal amino acids results in inactive enzyme, while 4 C-terminal residues can be deleted without losing enzyme activity	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
[ATP-citrate lyase]-N-phospho-L-histidine + H2O	Homo sapiens	-	[ATP-citrate lyase]-L-histidine + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography, and gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[ATP-citrate lyase]-N-phospho-L-histidine + H2O	-	Homo sapiens	[ATP-citrate lyase]-L-histidine + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
PHP	-	Homo sapiens
protein histidine phosphatase	-	Homo sapiens

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Release 2023.1 (January 2023)