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Literature summary for 3.8.1.7 extracted from

  • Zhou, L.; Poh, R.P.; Marks, T.S.; Chowdhry, B.Z.; Smith, A.R.
    Structure and denaturation of 4-chlorobenzoyl coenzyme A dehalogenase from Arthrobacter sp. strain TM-1 (2008), Biodegradation, 19, 65-75.
    View publication on PubMed
Search for more literature for 3.8.1.7

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-chlorobenzoyl-CoA + H2O Arthrobacter sp.
-
 4-hydroxybenzoyl-CoA + chloride
-
 ?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
 Arthrobacter sp. strain TM-1
-

Purification (Commentary)

Purification (Comment) Organism
4-CBCoA dehalogenase is purified to homogeneity from cells of Arthrobacter sp. strain TM-1 Arthrobacter sp.

Renatured (Commentary)

Renatured (Comment) Organism
denaturation and renaturation experiments as functions of pH and temperature are performed Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.007
-
 time elapsed at 45°C, 1020 min, recovery 1.7% Arthrobacter sp.
0.08
-
 time elapsed at 45°C, 120 min, recovery 19.9% Arthrobacter sp.
0.082
-
 time elapsed at 45°C, 0 min, recovery 20.4% Arthrobacter sp.
0.082
-
 time elapsed at 45°C, 15 min, recovery 20.4% Arthrobacter sp.
0.082
-
 time elapsed at 45°C, 5 min, recovery 20.4% Arthrobacter sp.
0.194
-
 renaturation of 4-CBCoA dehalogenaase following exposure to elevated temperature, 55°C, activity after heating to 60°C 0.081, recovery 20.1% Arthrobacter sp.
0.243
-
 renaturation of 4-CBCoA dehalogenaase following exposure to elevated temperature, 20°C, activity after heating to 60°C 0.038, recovery 9.5% Arthrobacter sp.
0.301
-
 renaturation of 4-CBCoA dehalogenaase following exposure to elevated temperature, 30°C, activity after heating to 60°C 0.051, recovery 12.7% Arthrobacter sp.
0.349
-
 renaturation of 4-CBCoA dehalogenaase following exposure to elevated temperature, 50°C, activity after heating to 60°C 0.082, recovery 20.4% Arthrobacter sp.
0.36
-
 renaturation of 4-CBCoA dehalogenaase following exposure to elevated temperature, 40°C, activity after heating to 60°C 0.055, recovery 13.7% Arthrobacter sp.
0.363
-
 renaturation of 4-CBCoA dehalogenaase after return from pH 5.2 to pH 7.4, incubation time 7 min, recovery 89% Arthrobacter sp.
0.365
-
 renaturation of 4-CBCoA dehalogenaase after return from pH 10.1 to pH 7.4, incubation time 7 min, recovery 90% Arthrobacter sp.
0.402
-
 renaturation of 4-CBCoA dehalogenaase following exposure to elevated temperature, 45°C, activity after heating to 60°C 0.083, recovery 20.6% Arthrobacter sp.
0.407
-
 renaturation of 4-CBCoA dehalogenaase after return from pH 10.1 to pH 7.4, incubation time 17 min, recovery 99% Arthrobacter sp.
0.41
-
 pH 7.4 Arthrobacter sp.
0.41
-
 renaturation of 4-CBCoA dehalogenaase after return from pH 5.2 to pH 7.4, incubation time 17 min, recovery 100% Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-chlorobenzoyl-CoA + H2O
-
 Arthrobacter sp. 4-hydroxybenzoyl-CoA + chloride
-
 ?

Subunits

Subunits Comment Organism
trimer
-
 Arthrobacter sp.

Synonyms

Synonyms Comment Organism
4-CBCoA dehalogenase
-
 Arthrobacter sp.
4-chlorobenzoyl-CoA dehalogenase
-
 Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 activity assay Arthrobacter sp.
45
-
 activity is maximal at 45°C and is totally lost by heating to 60°C for 5 min Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 activity assay Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
7.4
-
 when incubated for 5 Min at 1.5 mg ml-1 in buffer at 5.2 or 10.1 enzyme activity is lost completely Arthrobacter sp.

Cofactor

Cofactor Comment Organism Structure
coenzyme A
-
 Arthrobacter sp.