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Literature summary for 3.8.1.7 extracted from

  • Zhou, L.; Marks, T.S.; Poh, R.P.; Smith, R.J.; Chowdhry, B.Z.; Smith, A.R.
    The purification and characterisation of 4-chlorobenzoate:CoA ligase and 4-chlorobenzoyl CoA dehalogenase from Arthrobacter sp. strain TM-1 (2004), Biodegradation, 15, 97-109.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
4-chlorobenzoyl-CoA 30°C, pH 7.5 Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
4 * 33000, SDS-PAGE Arthrobacter sp.
131000
-
gel filtration Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-
Arthrobacter sp. TM-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.61
-
pH 7.5, 30°C Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-chlorobenzoyl-CoA + H2O
-
Arthrobacter sp. 4-hydroxybenzoyl-CoA + chloride
-
?
4-chlorobenzoyl-CoA + H2O
-
Arthrobacter sp. TM-1 4-hydroxybenzoyl-CoA + chloride
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 33000, SDS-PAGE Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Arthrobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 55 15°C: about 50% of maximal activity, 55°C: about 50% of maximal activity Arthrobacter sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
4-chlorobenzoyl-CoA 30°C, pH 7.5 Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 9.3 pH 6.0: about 55% of maximal activity, pH 9.3: about 55% of maximal activity Arthrobacter sp.

pI Value

Organism Comment pI Value Maximum pI Value
Arthrobacter sp. isoelectric focusing
-
6.4