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Literature summary for 3.8.1.5 extracted from

  • Erable, B.; Goubet, I.; Lamare, S.; Legoy, M.D.; Maugard, T.
    Haloalkane hydrolysis by Rhodococcus erythropolis cells: comparison of conventional aqueous phase dehalogenation and nonconventional gas phase dehalogenation (2004), Biotechnol. Bioeng., 86, 47-54.
    View publication on PubMed

Application

Application Comment Organism
biotechnology haloalkane hydrolysis by lyophilized cells in solid/gas biofilter and in the aqueous phase. Comparison of substrates Rhodococcus erythropolis
biotechnology hydrolysis of haloalkanes by lyophilized cells in solid-gas biofilter and aqueous phase. For both systems, pH 9.0 and 40°C are the best conditions. In the aqueous phase, cells are less sensitive to variation in pH -value than in gas phase Rhodococcus erythropolis

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis
-
induction by growth in minimal salt medium and by addition of up to 0.05% 1-chlorobutane to medium
-
Rhodococcus erythropolis
-
lyophilized cells
-
Rhodococcus erythropolis NCIMB 13064
-
lyophilized cells
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-bromobutane + H2O reaction in aqueous phase, 98% of the activity with 1-chlorobutane, reaction in gaseous phase, 98% of the activity with 1-chlorobutane Rhodococcus erythropolis butanol + bromide
-
?
1-bromobutane + H2O reaction in aqueous phase, 98% of the activity with 1-chlorobutane, reaction in gaseous phase, 98% of the activity with 1-chlorobutane Rhodococcus erythropolis NCIMB 13064 butanol + bromide
-
?
1-bromohexane + H2O reaction in aqueous phase, 87% of the activity with 1-chlorobutane, reaction in gaseous phase, 301% of the activity with 1-chlorobutane Rhodococcus erythropolis hexanol + bromide
-
?
1-chlorobutane + H2O
-
Rhodococcus erythropolis butanol + chloride
-
?
1-chlorobutane + H2O
-
Rhodococcus erythropolis NCIMB 13064 butanol + chloride
-
?
1-chlorohexane + H2O reaction in aqueous phase, 100% of the activity with 1-chlorobutane, reaction in gaseous phase, 194% of the activity with 1-chlorobutane Rhodococcus erythropolis hexanol + chloride
-
?
1-chlorohexane + H2O reaction in aqueous phase, 100% of the activity with 1-chlorobutane, reaction in gaseous phase, 194% of the activity with 1-chlorobutane Rhodococcus erythropolis NCIMB 13064 hexanol + chloride
-
?
1-chloropentane + H2O reaction in aqueous phase, 101% of the activity with 1-chlorobutane, reaction in gaseous phase, 186% of the activity with 1-chlorobutane Rhodococcus erythropolis pentanol + chloride
-
?
1-chloropentane + H2O reaction in aqueous phase, 101% of the activity with 1-chlorobutane, reaction in gaseous phase, 186% of the activity with 1-chlorobutane Rhodococcus erythropolis NCIMB 13064 pentanol + chloride
-
?
additional information no substrate: 1-chlorodecane, 2-chlorobutane Rhodococcus erythropolis ?
-
?
additional information no substrate: 1-chlorodecane, 2-chlorobutane Rhodococcus erythropolis NCIMB 13064 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
assay at Rhodococcus erythropolis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Rhodococcus erythropolis

pH Range

pH Minimum pH Maximum Comment Organism
9
-
hydrolysis of haloalkanes both in solid-gas biofilter and in the aqueous phase Rhodococcus erythropolis