Crystallization (Comment) | Organism |
---|---|
12 mg/ml purified recombinant His-tagged enzyme, effective crystallization conditions are not same as for the nontagged enzyme, hanging drop vapour diffusion method, 200 mM MgCl2, 100 mM Tris-HCl, pH 8.5, 20% w/v PEG 4000, equal volumes of 0.001 ml of protein and well solution are mixed on siliconized cover slips, 3 days, crystals are used for seeding, another 3 days, X-ray diffraction structure determination and analysis at 0.95 A resolution, molecular dynamics simulation | Sphingomonas paucimobilis |
side-chain of catalytic nucleophile N108 displays a high degree of disorder | Sphingomonas paucimobilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000 | Sphingomonas paucimobilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas paucimobilis | - |
- |
- |
Sphingomonas paucimobilis | - |
enzyme LinB | - |
Sphingomonas paucimobilis UT26 | - |
- |
- |
Sphingomonas paucimobilis UT26 | - |
enzyme LinB | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1-haloalkane + H2O = a primary alcohol + halide | catalytic nucleophile Asp108, which is the most disordered catalytic residue, catalytic base His272, structure-based reaction mechanism, active site structure, overview | Sphingomonas paucimobilis | |
1-haloalkane + H2O = a primary alcohol + halide | H272 singly protonated at Ndelta1 and D108 in conformation A give the most exothermic reactionwith DeltaH of -22 kcal/mol | Sphingomonas paucimobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-chloro-2,2-dimethylpropane + H2O | - |
Sphingomonas paucimobilis | 2,2-dimethylpropan-1-ol + chloride | - |
? | |
1-chloro-2,2-dimethylpropane + H2O | - |
Sphingomonas paucimobilis UT26 | 2,2-dimethylpropan-1-ol + chloride | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000 | Sphingomonas paucimobilis |
More | 2 enzyme conformations A and B | Sphingomonas paucimobilis |
Synonyms | Comment | Organism |
---|---|---|
LinB | - |
Sphingomonas paucimobilis |