Application | Comment | Organism |
---|---|---|
environmental protection | enzyme might be utilized for bioremediation of organohalide-contaminated industrial waste | Sphingomonas paucimobilis |
Crystallization (Comment) | Organism |
---|---|
crystallization of purified recombinant enzyme by hanging drop vapour diffusion method, from 18% PEG 6000, 0.2 M calcium acetate, and 0.1 M Tris, pH 8.9, determination of enzyme ligand complexe structures with substrates 1-chlorobutane, 1,2-dichloroethane, and 1,2-dichloropropane, X-ray diffraction structure determination and analysis at 1.8-1.9 A resolution | Sphingomonas paucimobilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,2-Dichloroethane | competitive to 1-chlorobutane | Sphingomonas paucimobilis | |
1,2-dichloropropane | competitive to 1-chlorobutane | Sphingomonas paucimobilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
1,2-Dichloroethane | pH 8.6, 37°C, recombinant enzyme | Sphingomonas paucimobilis | |
0.26 | - |
1,2-dichloropropane | pH 8.6, 37°C, recombinant enzyme | Sphingomonas paucimobilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas paucimobilis | - |
enzyme LinB | - |
Sphingomonas paucimobilis UT26 | - |
enzyme LinB | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1-haloalkane + H2O = a primary alcohol + halide | low activity with small, chlorinated alkanes, importance of active site water molecules and reaction products in molecular docking | Sphingomonas paucimobilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0007 | - |
substrate 1,2-dichloroethane | Sphingomonas paucimobilis |
0.0016 | - |
substrate 1,2-dichloropropane | Sphingomonas paucimobilis |
4.09 | - |
substrate 1-chlorobutane | Sphingomonas paucimobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dichloroethane + H2O | low activity | Sphingomonas paucimobilis | 2-chloroethanol + chloride | - |
? | |
1,2-dichloroethane + H2O | low activity | Sphingomonas paucimobilis UT26 | 2-chloroethanol + chloride | - |
? | |
1,2-dichloropropane + H2O | low activity | Sphingomonas paucimobilis | 2-chloropropan-1-ol + chloride | - |
? | |
1,2-dichloropropane + H2O | low activity | Sphingomonas paucimobilis UT26 | 2-chloropropan-1-ol + chloride | - |
? | |
1-chlorobutane + H2O | - |
Sphingomonas paucimobilis | n-butanol + chloride | - |
? | |
1-chlorobutane + H2O | - |
Sphingomonas paucimobilis UT26 | n-butanol + chloride | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LinB | - |
Sphingomonas paucimobilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sphingomonas paucimobilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.91 | - |
1,2-dichloropropane | pH 8.6, 37°C, recombinant enzyme | Sphingomonas paucimobilis | |
1.05 | - |
1,2-Dichloroethane | pH 8.6, 37°C, recombinant enzyme | Sphingomonas paucimobilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
assay at | Sphingomonas paucimobilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Sphingomonas paucimobilis | |
0.97 | - |
1,2-dichloropropane | pH 8.6, 37°C, recombinant enzyme | Sphingomonas paucimobilis | |
2.31 | - |
1,2-Dichloroethane | pH 8.6, 37°C, recombinant enzyme | Sphingomonas paucimobilis |