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Literature summary for 3.8.1.5 extracted from

  • Oakley, A.J.; Prokop, Z.; Bohac, M.; Kmunicek, J.; Jedlicka, T.; Monincova, M.; Kuta-Smatanova, I.; Nagata, Y.; Damborsky, J.; Wilce, M.C.J.
    Exploring the structure and activity of haloalkane dehalogenase from Sphingomonas paucimobilis UT26: evidence for product- and water-mediated inhibition (2002), Biochemistry, 41, 4847-4855.
    View publication on PubMed

Application

Application Comment Organism
environmental protection enzyme might be utilized for bioremediation of organohalide-contaminated industrial waste Sphingomonas paucimobilis

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization of purified recombinant enzyme by hanging drop vapour diffusion method, from 18% PEG 6000, 0.2 M calcium acetate, and 0.1 M Tris, pH 8.9, determination of enzyme ligand complexe structures with substrates 1-chlorobutane, 1,2-dichloroethane, and 1,2-dichloropropane, X-ray diffraction structure determination and analysis at 1.8-1.9 A resolution Sphingomonas paucimobilis

Inhibitors

Inhibitors Comment Organism Structure
1,2-Dichloroethane competitive to 1-chlorobutane Sphingomonas paucimobilis
1,2-dichloropropane competitive to 1-chlorobutane Sphingomonas paucimobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
1,2-Dichloroethane pH 8.6, 37°C, recombinant enzyme Sphingomonas paucimobilis
0.26
-
1,2-dichloropropane pH 8.6, 37°C, recombinant enzyme Sphingomonas paucimobilis

Organism

Organism UniProt Comment Textmining
Sphingomonas paucimobilis
-
enzyme LinB
-
Sphingomonas paucimobilis UT26
-
enzyme LinB
-

Reaction

Reaction Comment Organism Reaction ID
1-haloalkane + H2O = a primary alcohol + halide low activity with small, chlorinated alkanes, importance of active site water molecules and reaction products in molecular docking Sphingomonas paucimobilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0007
-
substrate 1,2-dichloroethane Sphingomonas paucimobilis
0.0016
-
substrate 1,2-dichloropropane Sphingomonas paucimobilis
4.09
-
substrate 1-chlorobutane Sphingomonas paucimobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dichloroethane + H2O low activity Sphingomonas paucimobilis 2-chloroethanol + chloride
-
?
1,2-dichloroethane + H2O low activity Sphingomonas paucimobilis UT26 2-chloroethanol + chloride
-
?
1,2-dichloropropane + H2O low activity Sphingomonas paucimobilis 2-chloropropan-1-ol + chloride
-
?
1,2-dichloropropane + H2O low activity Sphingomonas paucimobilis UT26 2-chloropropan-1-ol + chloride
-
?
1-chlorobutane + H2O
-
Sphingomonas paucimobilis n-butanol + chloride
-
?
1-chlorobutane + H2O
-
Sphingomonas paucimobilis UT26 n-butanol + chloride
-
?

Synonyms

Synonyms Comment Organism
LinB
-
Sphingomonas paucimobilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Sphingomonas paucimobilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.91
-
1,2-dichloropropane pH 8.6, 37°C, recombinant enzyme Sphingomonas paucimobilis
1.05
-
1,2-Dichloroethane pH 8.6, 37°C, recombinant enzyme Sphingomonas paucimobilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
assay at Sphingomonas paucimobilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics Sphingomonas paucimobilis
0.97
-
1,2-dichloropropane pH 8.6, 37°C, recombinant enzyme Sphingomonas paucimobilis
2.31
-
1,2-Dichloroethane pH 8.6, 37°C, recombinant enzyme Sphingomonas paucimobilis