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Literature summary for 3.8.1.5 extracted from

  • Krooshof, G.H.; Ridder, I.S.; Tepper, A.W.J.; Vos, G.J.; Rozeboom, H.J.; Kalk, K.H.; Dijkstra, B.W.; Janssen, D.B.
    Kinetic analysis and X-ray structure of haloalkane dehalogenase with a modified halide-binding site (1998), Biochemistry, 37, 15013-15023.
    View publication on PubMed
Search for more literature for 3.8.1.5

Crystallization (Commentary)

Crystallization (Comment) Organism
GJ10, X-ray structure at pH 6.2 and pH 5.0 at room temperature of wild-type and mutant W175Y Xanthobacter autotrophicus

Protein Variants

Protein Variants Comment Organism
W175Y
-
 Xanthobacter autotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 1,2-Dibromoethane GJ10 mutant W175Y Xanthobacter autotrophicus
0.53
-
 1,2-Dichloroethane GJ10 Xanthobacter autotrophicus
2.85
-
 1,2-Dichloroethane GJ10 mutant W175Y Xanthobacter autotrophicus
5.6
-
 1,2-Dibromoethane GJ10, calculated from rate constants Xanthobacter autotrophicus
10
-
 1,2-Dibromoethane GJ10, steady state experiments Xanthobacter autotrophicus
59
-
 1,2-Dibromoethane GJ10mutant W175Y, calculated from rate constants Xanthobacter autotrophicus
60
-
 1,2-Dibromoethane GJ10 mutant W175Y, steady state experiments Xanthobacter autotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
n-haloalkanes + H2O Xanthobacter autotrophicus broad substrate range primary alcohols + halide
-
 ?
n-haloalkanes + H2O Xanthobacter autotrophicus GJ10 broad substrate range primary alcohols + halide
-
 ?

Organism

Organism UniProt Comment Textmining
Xanthobacter autotrophicus P22643 GJ10
-
Xanthobacter autotrophicus GJ10 P22643 GJ10
-

Purification (Commentary)

Purification (Comment) Organism
GJ10 Xanthobacter autotrophicus

Reaction

Reaction Comment Organism Reaction ID
1-haloalkane + H2O = a primary alcohol + halide GJ10, three-step reaction mechanism with covalent alkyl-enzyme ester intermediate Xanthobacter autotrophicus
a
1-haloalkane + H2O = a primary alcohol + halide alpha/beta hydrolase with catalytic triad, i.e. nucleophile (Asp124)-histidine (H289)-acid (Asp260), during catalytic reaction Xanthobacter autotrophicus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 profound kinetic calculations Xanthobacter autotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dibromoethane + H2O
-
 Xanthobacter autotrophicus 2-bromoethanol + bromide
-
 ?
1,2-dibromoethane + H2O
-
 Xanthobacter autotrophicus GJ10 2-bromoethanol + bromide
-
 ?
1,2-dichloroethane + H2O
-
 Xanthobacter autotrophicus 2-chloroethanol + chloride
-
 ?
1,2-dichloroethane + H2O
-
 Xanthobacter autotrophicus GJ10 2-chloroethanol + chloride
-
 ?
haloalkane + H2O
-
 Xanthobacter autotrophicus alcohol + halide
-
 ?
haloalkane + H2O
-
 Xanthobacter autotrophicus GJ10 alcohol + halide
-
 ?
n-haloalkanes + H2O broad substrate range Xanthobacter autotrophicus primary alcohols + halide
-
 ?
n-haloalkanes + H2O broad substrate range Xanthobacter autotrophicus GJ10 primary alcohols + halide
-
 ?