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Literature summary for 3.8.1.5 extracted from

  • Keuning, S.; Janssen, D.B.; Witholt, B.
    Purification and characterization of hydrolytic haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 (1985), J. Bacteriol., 163, 635-639.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
degradation simple route of detoxification Xanthobacter autotrophicus

Inhibitors

Inhibitors Comment Organism Structure
HgCl2
-
Xanthobacter autotrophicus
iodoacetamide
-
Xanthobacter autotrophicus
NEM
-
Xanthobacter autotrophicus
PCMB
-
Xanthobacter autotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
1,2-Dichloroethane
-
Xanthobacter autotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
gel filtration Xanthobacter autotrophicus
36000
-
1 * 36000, SDS-PAGE Xanthobacter autotrophicus

Organism

Organism UniProt Comment Textmining
Xanthobacter autotrophicus
-
GJ10
-
Xanthobacter autotrophicus GJ10
-
GJ10
-

Purification (Commentary)

Purification (Comment) Organism
GJ10 Xanthobacter autotrophicus

Storage Stability

Storage Stability Organism
4°C, 50 mM Tris-sulfate buffer, pH 7.5, 1 mM EDTA, 1 mM beta-mercaptoethanol, less than 20% loss of activity after several weeks Xanthobacter autotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dibromoethane + H2O 94% of the activity with 1,2-dichloroethane Xanthobacter autotrophicus 2-bromoethanol + bromide
-
?
1,2-dichloroethane + H2O highest activity Xanthobacter autotrophicus 2-chloroethanol + chloride
-
?
1,2-dichloroethane + H2O highest activity Xanthobacter autotrophicus GJ10 2-chloroethanol + chloride
-
?
1,3-dichloropropane + H2O 80% of the activity with 1,2-dichloroethane Xanthobacter autotrophicus ?
-
?
1-bromopropane + H2O 29% of the activity with 1,2-dichloroethane Xanthobacter autotrophicus propanol + bromide
-
?
1-chlorobutane + H2O 31% of the activity with 1,2-dichloroethane Xanthobacter autotrophicus butanol + chloride
-
?
1-chloropropane + H2O 51% of the activity with 1,2-dichloroethane Xanthobacter autotrophicus propanol + chloride
-
?
1-chloropropane + H2O 51% of the activity with 1,2-dichloroethane Xanthobacter autotrophicus GJ10 propanol + chloride
-
?
1-iodopropane + H2O 14% of the activity with 1,2-dichloroethane Xanthobacter autotrophicus propanol + iodide
-
?
3-chloropropene + H2O 45% of the activity with 1,2-dichloroethane Xanthobacter autotrophicus propenol + chloride
-
?
bromoethane + H2O 24% of the activity with 1,2-dichloroethane Xanthobacter autotrophicus ethanol + bromide
-
?
chloroethane + H2O 24% of the activity with 1,2-dichloroethane Xanthobacter autotrophicus ethanol + chloride
-
?
chloroethane + H2O 24% of the activity with 1,2-dichloroethane Xanthobacter autotrophicus GJ10 ethanol + chloride
-
?
chloromethane + H2O 28% of the activity with 1,2-dichloroethane Xanthobacter autotrophicus methanol + chloride
-
?
chloromethane + H2O 28% of the activity with 1,2-dichloroethane Xanthobacter autotrophicus GJ10 methanol + chloride
-
?
haloalkane + H2O n-halogenated C1 to C4 alkanes, including chlorinated, brominated and iodinated compounds Xanthobacter autotrophicus alcohol + halide
-
?
haloalkane + H2O n-halogenated C1 to C4 alkanes, including chlorinated, brominated and iodinated compounds Xanthobacter autotrophicus GJ10 alcohol + halide
-
?

Subunits

Subunits Comment Organism
monomer 1 * 36000, SDS-PAGE Xanthobacter autotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Xanthobacter autotrophicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
inactivation above 50°C Xanthobacter autotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
Xanthobacter autotrophicus