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Literature summary for 3.8.1.3 extracted from

  • Liu, J.Q.; Kurihara, T.; Ichiyama, S.; Miyagi, M.; Tsunasawa, S.; Kawasaki, H.; Soda, K.; Esaki, N.
    Reaction mechanism of fluoroacetate dehalogenase from Moraxella sp. B (1998), J. Biol. Chem., 47, 30897-30902.
No PubMed abstract available
Search for more literature for 3.8.1.3

Application

Application Comment Organism
agriculture detoxification of poisonous plants for animal food Moraxella sp.

Cloned(Commentary)

Cloned (Comment) Organism
mutant expression in Escherichia coli, H1 Moraxella sp.

Protein Variants

Protein Variants Comment Organism
H272N mutant, no activity, substrate blocks binding pocket Moraxella sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33258
-
 2 * 33258, H1 Moraxella sp.
67000
-
 gel filtration Moraxella sp.
67000
-
 H1 recombinant in E. coli Moraxella sp.

Organism

Organism UniProt Comment Textmining
Moraxella sp.
-
-
-

Subunits

Subunits Comment Organism
dimer 2 * 33258, H1 Moraxella sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 recombinant in E.coli Moraxella sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 60 70% of initial activity lost after 30 min at 50°C Moraxella sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10
-
 Moraxella sp.