Application | Comment | Organism |
---|---|---|
analysis | the enzyme is covalently linked to an N-hydroxysuccinimidyl Sepharose resin to construct a highly specific sensor with long shelf life for the detection of L-2-haloacids | Sulfurisphaera tokodaii |
General Stability | Organism |
---|---|
the enzyme retains approximately 30% of the overall activity after immobilization | Sulfurisphaera tokodaii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
(S)-2-chloropropionic acid | pH 9.0, 50°C, soluble enzyme | Sulfurisphaera tokodaii | |
1.6 | - |
(S)-2-chloropropionic acid | pH 9.0, 22°C, immobilized enzyme | Sulfurisphaera tokodaii | |
2.7 | - |
(S)-2-chloropropionic acid | pH 9.0, 37°C, immobilized enzyme | Sulfurisphaera tokodaii | |
4.9 | - |
(S)-2-chloropropionic acid | pH 9.0, 50°C, immobilized enzyme | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfurisphaera tokodaii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.9 | - |
substrate: (S)-2-chloropropionic acid, pH 9.0, 22°C, immobilized enzyme | Sulfurisphaera tokodaii |
3 | - |
substrate: (S)-2-chloropropionic acid, pH 9.0, 37°C, immobilized enzyme | Sulfurisphaera tokodaii |
6.3 | - |
substrate: (S)-2-chloropropionic acid, pH 9.0, 50°C, immobilized enzyme | Sulfurisphaera tokodaii |
20 | - |
substrate: (S)-2-chloropropionic acid, pH 9.0, 50°C, soluble enzyme | Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-chloropropionic acid + H2O | - |
Sulfurisphaera tokodaii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-HADST | - |
Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
soluble and immobilized enzyme | Sulfurisphaera tokodaii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10.5 | pH 8.0: about 50% of maximal activity, pH 10.5: about 80% of maximal activity | Sulfurisphaera tokodaii |