Crystallization (Comment) | Organism |
---|---|
A129V mutant, at 1.65 A resolution, crystals of the A129V mutant isomorphous with the S103A crystals | Pseudomonas fluorescens |
Protein Variants | Comment | Organism |
---|---|---|
A129V | improved catalytic efficiency than the wild type enzyme, possesses a more appropiate binding pocket for substrates with smaller C6 substituents | Pseudomonas fluorescens |
A129V/I199V/V227I | combination of mutations incompatible for correct folding | Pseudomonas fluorescens |
F104M | mutation does not have any significant effect on enzyme characteristics | Pseudomonas fluorescens |
I199V | improved catalytic efficiency than the wild type enzyme | Pseudomonas fluorescens |
I199V/V2271 | combination of mutations incompatible for correct folding | Pseudomonas fluorescens |
S34A | lower Km and turnover rates compared to wild-type | Pseudomonas fluorescens |
S34G | lower Km and turnover rates compared to wild-type | Pseudomonas fluorescens |
V227I | improved catalytic efficiency than the wild type enzyme | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00045 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | mutant I199V | Pseudomonas fluorescens | |
0.0008 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | mutant S34G | Pseudomonas fluorescens | |
0.0014 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | mutant I199V | Pseudomonas fluorescens | |
0.0017 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | mutant A129/I199V | Pseudomonas fluorescens | |
0.0018 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | mutant A129V | Pseudomonas fluorescens | |
0.002 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | mutant V227I | Pseudomonas fluorescens | |
0.0022 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | wild-type | Pseudomonas fluorescens | |
0.0023 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | mutant I199V | Pseudomonas fluorescens | |
0.0024 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | mutant A129/V227I | Pseudomonas fluorescens | |
0.0025 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | mutant S34A | Pseudomonas fluorescens | |
0.0026 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | mutant S34G | Pseudomonas fluorescens | |
0.0027 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | mutant F104M | Pseudomonas fluorescens | |
0.0028 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | mutant V227I | Pseudomonas fluorescens | |
0.0031 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | mutant S34A | Pseudomonas fluorescens | |
0.0035 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | mutant A129/I199V | Pseudomonas fluorescens | |
0.0035 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | mutant I199V | Pseudomonas fluorescens | |
0.0039 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | mutant F104M | Pseudomonas fluorescens | |
0.004 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | mutant F104M | Pseudomonas fluorescens | |
0.0042 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | mutant S34G | Pseudomonas fluorescens | |
0.0048 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | mutant A129/I199V | Pseudomonas fluorescens | |
0.0049 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | mutant V227I | Pseudomonas fluorescens | |
0.0049 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | mutant V227I | Pseudomonas fluorescens | |
0.005 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | mutant A129/I199V | Pseudomonas fluorescens | |
0.005 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | mutant S34A | Pseudomonas fluorescens | |
0.0055 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | mutant A129/V227I | Pseudomonas fluorescens | |
0.0056 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | mutant A129V | Pseudomonas fluorescens | |
0.0058 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | mutant A129/V227I | Pseudomonas fluorescens | |
0.0078 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | wild-type | Pseudomonas fluorescens | |
0.0089 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | wild-type | Pseudomonas fluorescens | |
0.0098 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | mutant A129V | Pseudomonas fluorescens | |
0.01 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | mutant A129/V227I | Pseudomonas fluorescens | |
0.01 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | mutant A129V | Pseudomonas fluorescens | |
0.011 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | wild-type | Pseudomonas fluorescens | |
0.015 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | - |
Pseudomonas putida | |
0.017 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | - |
Pseudomonas putida | |
0.022 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | - |
Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
IP01 | - |
Pseudomonas fluorescens IP01 | - |
IP01 | - |
Pseudomonas putida | - |
F1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
Pseudomonas fluorescens | ? | - |
? | |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
Pseudomonas fluorescens IP01 | ? | - |
? | |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O | - |
Pseudomonas fluorescens | ? | - |
? | |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O | - |
Pseudomonas putida | ? | - |
? | |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O | - |
Pseudomonas fluorescens IP01 | ? | - |
? | |
2-hydroxy-6-oxohepta-2,4-dienoate + H2O | - |
Pseudomonas fluorescens | ? | - |
? | |
2-hydroxy-6-oxohepta-2,4-dienoate + H2O | - |
Pseudomonas putida | ? | - |
? | |
2-hydroxy-6-oxohepta-2,4-dienoate + H2O | - |
Pseudomonas fluorescens IP01 | ? | - |
? | |
2-hydroxy-6-oxoocta-2,4-dienoate + H2O | - |
Pseudomonas fluorescens | ? | - |
? | |
2-hydroxy-6-oxoocta-2,4-dienoate + H2O | - |
Pseudomonas putida | ? | - |
? | |
2-hydroxy-6-oxoocta-2,4-dienoate + H2O | - |
Pseudomonas fluorescens IP01 | ? | - |
? | |
additional information | can not hydrolyze 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | Pseudomonas fluorescens | ? | - |
? | |
additional information | can not hydrolyze 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | Pseudomonas putida | ? | - |
? | |
additional information | can not hydrolyze 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | Pseudomonas fluorescens IP01 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CumD | - |
Pseudomonas fluorescens |
meta-cleavage product hydrolase | - |
Pseudomonas fluorescens |
meta-cleavage product hydrolase | - |
Pseudomonas putida |
TodF | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | mutant A129/V227I | Pseudomonas fluorescens | |
0.01 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | mutant A129/I199V | Pseudomonas fluorescens | |
0.01 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | mutant A129V | Pseudomonas fluorescens | |
0.02 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | mutant I199V | Pseudomonas fluorescens | |
0.02 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | mutant V227I | Pseudomonas fluorescens | |
0.037 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | wild-type | Pseudomonas fluorescens | |
0.3 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | mutant S34A | Pseudomonas fluorescens | |
0.41 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | - |
Pseudomonas putida | |
0.73 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | mutant A129/V227I | Pseudomonas fluorescens | |
1.3 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | mutant S34A | Pseudomonas fluorescens | |
1.5 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | mutant S34G | Pseudomonas fluorescens | |
3.3 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | mutant A129/I199V | Pseudomonas fluorescens | |
4.55 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | mutant A129/I199V | Pseudomonas fluorescens | |
6.4 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | mutant F104M | Pseudomonas fluorescens | |
6.5 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | mutant F104M | Pseudomonas fluorescens | |
6.7 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | mutant S34A | Pseudomonas fluorescens | |
8.1 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | mutant S34G | Pseudomonas fluorescens | |
8.4 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | mutant S34G | Pseudomonas fluorescens | |
14 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | - |
Pseudomonas putida | |
15.4 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | mutant F104M | Pseudomonas fluorescens | |
19 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | mutant A129/I199V | Pseudomonas fluorescens | |
19.5 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | wild-type | Pseudomonas fluorescens | |
20 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | mutant V227I | Pseudomonas fluorescens | |
23.1 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | wild-type | Pseudomonas fluorescens | |
24 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | mutant A129/V227I | Pseudomonas fluorescens | |
25 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | mutant A129V | Pseudomonas fluorescens | |
29 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | mutant A129/V227I | Pseudomonas fluorescens | |
29 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | mutant I199V | Pseudomonas fluorescens | |
35 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | - |
Pseudomonas putida | |
35 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | mutant A129/I199V | Pseudomonas fluorescens | |
37 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | mutant I199V | Pseudomonas fluorescens | |
38 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | mutant I199V | Pseudomonas fluorescens | |
42.4 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | wild-type | Pseudomonas fluorescens | |
52 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | mutant V227I | Pseudomonas fluorescens | |
75 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | mutant A129V | Pseudomonas fluorescens | |
78 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | mutant V227I | Pseudomonas fluorescens | |
81 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | mutant A129V | Pseudomonas fluorescens |