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Literature summary for 3.7.1.7 extracted from

  • Bennett, J.P.; Whittingham, J.L.; Brzozowski, A.M.; Leonard, P.M.; Grogan, G.
    Structural characterization of a beta-diketone hydrolase from the cyanobacterium Anabaena sp. PCC 7120 in native and product-bound forms, a coenzyme A-independent member of the crotonase suprafamily (2007), Biochemistry, 46, 137-144.
    View publication on PubMed
Search for more literature for 3.7.1.7

Cloned(Commentary)

Cloned (Comment) Organism
into the pETYSBLIC vector for expression in Escherichia coli BL21DE3 cells Nostoc sp. PCC 7120 = FACHB-418

Crystallization (Commentary)

Crystallization (Comment) Organism
the structure of beta-diketone hydrolase is determined to a resolution of 1.5 A in both native and ligand-bound forms Nostoc sp. PCC 7120 = FACHB-418

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000044
-
 bicyclo[2.2.2]octane-2,6-dione
-
 Nostoc sp. PCC 7120 = FACHB-418

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28800
-
 predicted molecular mass Nostoc sp. PCC 7120 = FACHB-418
30000
-
 determined by SDS-PAGE Nostoc sp. PCC 7120 = FACHB-418

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nonane-4,6-dione + H2O Nostoc sp. PCC 7120 = FACHB-418
-
 pentan-2-one + butanoate
-
 ?

Organism

Organism UniProt Comment Textmining
Nostoc sp. PCC 7120 = FACHB-418 Q8YNV6
-
-

Purification (Commentary)

Purification (Comment) Organism
using a nickel affinity HiTRAP agarose column and a S200 16/60 Sephadex column Nostoc sp. PCC 7120 = FACHB-418

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bicyclo[2.2.2]octane-2,6-dione + H2O desymmetrization of bicyclo[2.2.2]octane-2,6-dione Nostoc sp. PCC 7120 = FACHB-418 [(S)-3-oxocyclohexyl]acetic acid
-
 ?
nonane-4,6-dione + H2O
-
 Nostoc sp. PCC 7120 = FACHB-418 pentan-2-one + butanoate
-
 ?

Subunits

Subunits Comment Organism
homohexamer
-
 Nostoc sp. PCC 7120 = FACHB-418

Synonyms

Synonyms Comment Organism
ABDH
-
 Nostoc sp. PCC 7120 = FACHB-418
Alr4455 protein
-
 Nostoc sp. PCC 7120 = FACHB-418
beta-diketone hydrolase
-
 Nostoc sp. PCC 7120 = FACHB-418

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.16
-
 bicyclo[2.2.2]octane-2,6-dione
-
 Nostoc sp. PCC 7120 = FACHB-418

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 retro-Claisenase activity assay Nostoc sp. PCC 7120 = FACHB-418