Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, the X-ray structure of a succinate-H263A MhpC complex shows concerted movements in the positions of both Phe173 and Trp264 that line the approach to Arg188 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C261A | 1.4fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | Escherichia coli |
F173D | 158fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | Escherichia coli |
F173G | 32fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | Escherichia coli |
N109A | 125fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | Escherichia coli |
N109H | 217fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | Escherichia coli |
R188K | 217fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | Escherichia coli |
R188Q | 2941fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | Escherichia coli |
W264G | 196fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme N109A | Escherichia coli | |
0.004 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme N109H | Escherichia coli | |
0.0042 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme C261A | Escherichia coli | |
0.0068 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°, wild-type enzyme | Escherichia coli | |
0.01 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme F173D | Escherichia coli | |
0.038 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme R188K | Escherichia coli | |
0.058 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme F173G | Escherichia coli | |
0.077 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme R188Q | Escherichia coli | |
0.125 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme W264G | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate + H2O | Escherichia coli | the enzyme is involved in 3-phenylpropanoate catabolism | (2E)-2-hydroxypenta-2,4-dienoate + succinate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P77044 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate + H2O | - |
Escherichia coli | (2E)-2-hydroxypenta-2,4-dienoate + succinate | - |
? | |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate + H2O | the enzyme is involved in 3-phenylpropanoate catabolism | Escherichia coli | (2E)-2-hydroxypenta-2,4-dienoate + succinate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase | - |
Escherichia coli |
MhpC | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme N109H | Escherichia coli | |
0.1 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme R188Q | Escherichia coli | |
0.13 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme N109A | Escherichia coli | |
0.26 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme F173D | Escherichia coli | |
0.74 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme R188K | Escherichia coli | |
2 | 8 | (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°, wild-type enzyme | Escherichia coli | |
2.7 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme W264G | Escherichia coli | |
7.5 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme F173G | Escherichia coli | |
12 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme C261A | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in 3-phenylpropanoate catabolism | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme R188Q | Escherichia coli | |
19 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme N109H | Escherichia coli | |
19 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme R188K | Escherichia coli | |
21 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme W264G | Escherichia coli | |
26 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme F173D | Escherichia coli | |
33 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme N109A | Escherichia coli | |
130 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme F173G | Escherichia coli | |
2900 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme C261A | Escherichia coli | |
4118 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°, wild-type enzyme | Escherichia coli |