Cloned (Comment) | Organism |
---|---|
into the vector pET-3c | Dianthus caryophyllus |
into the vector pET-3c | Botrytis cinerea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
(2R,3S)-2,3-dimethylmalate | mutant S260P | Botrytis cinerea | |
0.054 | - |
(2R,3S)-2,3-dimethylmalate | mutant S260A | Botrytis cinerea | |
0.065 | - |
oxaloacetate | wild-type protein | Botrytis cinerea | |
0.13 | - |
oxaloacetate | wild-type protein | Dianthus caryophyllus | |
0.14 | - |
(2R,3S)-2,3-dimethylmalate | wild-type protein | Botrytis cinerea | |
0.15 | - |
oxaloacetate | mutant S260P, trial 1 | Botrytis cinerea | |
0.22 | - |
oxaloacetate | mutant S260P, trial 2 | Botrytis cinerea | |
0.53 | - |
(2R,3S)-2-ethyl-3-methylmalate | wild-type protein | Dianthus caryophyllus | |
0.56 | - |
(2R,3S)-2-ethyl-3-methylmalate | mutant S257A | Dianthus caryophyllus | |
0.66 | - |
(2R,3S)-2-ethyl-3-methylmalate | mutant S257P | Dianthus caryophyllus | |
0.7 | - |
oxaloacetate | mutant S260A, trial 1 | Botrytis cinerea | |
0.84 | - |
oxaloacetate | mutant S257P | Dianthus caryophyllus | |
1.307 | - |
oxaloacetate | mutant S260A, trial 2 | Botrytis cinerea | |
1.53 | - |
oxaloacetate | mutant S257A | Dianthus caryophyllus | |
2.6 | - |
oxaloacetate | mutant S257T, trial 2 | Dianthus caryophyllus | |
3.6 | - |
oxaloacetate | mutant S257T, trial 1 | Dianthus caryophyllus | |
7 | - |
oxaloacetate | mutant S260T, trial 1 | Botrytis cinerea | |
7.7 | - |
oxaloacetate | mutant S260T, trial 2 | Botrytis cinerea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-2,3-dimethylmalate | Botrytis cinerea | - |
pyruvate + propionic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Botrytis cinerea | Q6PNM8 | - |
- |
Dianthus caryophyllus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
proteins are purified to homogeneity | Dianthus caryophyllus |
proteins are purified to homogeneity | Botrytis cinerea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-2,3-dimethylmalate | - |
Botrytis cinerea | pyruvate + propionic acid | - |
? | |
(2R,3S)-2-ethyl-3-methylmalate + H2O | - |
Dianthus caryophyllus | ? | - |
? | |
oxaloacetate + H2O | - |
Dianthus caryophyllus | oxalate + acetate | - |
? | |
oxaloacetate + H2O | - |
Botrytis cinerea | oxalate + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OAH | - |
Botrytis cinerea |
OAH-active petal death protein | - |
Dianthus caryophyllus |
oxaloacetase | - |
Botrytis cinerea |
oxaloacetate acetylhydrolase | - |
Botrytis cinerea |
oxaloacetate hydrolase | - |
Botrytis cinerea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
activity assay | Dianthus caryophyllus |
25 | - |
activity assay | Botrytis cinerea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0249 | - |
(2R,3S)-2,3-dimethylmalate | mutant S260A | Botrytis cinerea | |
0.0293 | - |
(2R,3S)-2,3-dimethylmalate | wild-type protein | Botrytis cinerea | |
0.074 | - |
(2R,3S)-2,3-dimethylmalate | mutant S260P | Botrytis cinerea | |
0.71 | - |
oxaloacetate | mutant S260A, trial 2 | Botrytis cinerea | |
0.83 | - |
oxaloacetate | mutant S260A, trial 1 | Botrytis cinerea | |
0.96 | - |
oxaloacetate | mutant S260P, trial 1 | Botrytis cinerea | |
0.98 | - |
oxaloacetate | mutant S260P, trial 2 | Botrytis cinerea | |
1.14 | - |
oxaloacetate | mutant S257T, trial 1 | Dianthus caryophyllus | |
1.16 | - |
oxaloacetate | mutant S257T, trial 2 | Dianthus caryophyllus | |
2.59 | - |
oxaloacetate | mutant S260T, trial 1 | Botrytis cinerea | |
2.72 | - |
oxaloacetate | wild-type protein | Dianthus caryophyllus | |
2.91 | - |
oxaloacetate | mutant S257A | Dianthus caryophyllus | |
3.6 | - |
oxaloacetate | mutant S260T, trial 2 | Botrytis cinerea | |
4.1 | - |
oxaloacetate | mutant S257P | Dianthus caryophyllus | |
5.4 | - |
(2R,3S)-2-ethyl-3-methylmalate | mutant S257A | Dianthus caryophyllus | |
8.3 | - |
(2R,3S)-2-ethyl-3-methylmalate | mutant S257P | Dianthus caryophyllus | |
8.4 | - |
(2R,3S)-2-ethyl-3-methylmalate | wild-type protein | Dianthus caryophyllus | |
17.4 | - |
oxaloacetate | wild-type protein | Botrytis cinerea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
activity assay | Dianthus caryophyllus |
7.5 | - |
activity assay | Botrytis cinerea |