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Literature summary for 3.7.1.1 extracted from

  • Han, Y.; Joosten, H.J.; Niu, W.; Zhao, Z.; Mariano, P.S.; McCalman, M.; van Kan, J.; Schaap, P.J.; Dunaway-Mariano, D.
    Oxaloacetate hydrolase, the C-C bond lyase of oxalate secreting fungi (2007), J. Biol. Chem., 282, 9581-9590.
    View publication on PubMed

Application

Application Comment Organism
food industry oxalate toxicity is a concern for the commercial application of fungi in the food and drug industries Botrytis cinerea
food industry oxalate toxicity is a concern for the commercial application of fungi in the food and drug industries Aspergillus niger

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pGEM-T Easy for sequencing Aspergillus niger
into the vector pGEM-T Easy for sequencing, and into pET-3c for expression in Escherichia coli BL21DE3 cells Botrytis cinerea

Inhibitors

Inhibitors Comment Organism Structure
3,3-difluorooxaloacetate
-
Aspergillus niger
3,3-difluorooxaloacetate
-
Botrytis cinerea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.062
-
oxaloacetate cofactor Mg2+, buffer imidazole Botrytis cinerea
0.064
-
oxaloacetate cofactor Mg2+, buffer Hepes Botrytis cinerea
0.084
-
oxaloacetate cofactor Mg2+, buffer Tris Botrytis cinerea
0.24
-
oxaloacetate cofactor Mn2+, buffer imidazole Botrytis cinerea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34360
-
theoretical molecular mass Botrytis cinerea
35000
-
determined by SDS-PAGE Botrytis cinerea
100000
-
determined by gel filtration, indicative of a homotrimeric quaternary structure Botrytis cinerea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxaloacetate + H2O Botrytis cinerea
-
oxalate + acetate
-
?
oxaloacetate + H2O Aspergillus niger
-
oxalate + acetate
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger Q7Z986
-
-
Botrytis cinerea Q6PNM8
-
-

Purification (Commentary)

Purification (Comment) Organism
by ammonium sulfate-induced protein precipitation, and by using of a DEAE-cellulose and a butyl-Sepharose column Botrytis cinerea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.62
-
cell extract Botrytis cinerea
4.6
-
purification step ammonium sulfate precipitation Botrytis cinerea
15
-
purification step DEAE-cellulose column Botrytis cinerea
20
-
purification step butyl-Sepharose column Botrytis cinerea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxaloacetate + H2O
-
Botrytis cinerea oxalate + acetate
-
?
oxaloacetate + H2O
-
Aspergillus niger oxalate + acetate
-
?

Subunits

Subunits Comment Organism
More the results are consistent with the existence of a monomer-dimer-tetramer equilibrium Botrytis cinerea

Synonyms

Synonyms Comment Organism
OAH
-
Botrytis cinerea
OAH
-
Aspergillus niger
oxaloacetate acetylhydrolase
-
Botrytis cinerea
oxaloacetate acetylhydrolase
-
Aspergillus niger
oxaloacetate hydrolase
-
Botrytis cinerea
oxaloacetate hydrolase
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
oxaloacetate hydrolase assay Botrytis cinerea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.35
-
oxaloacetate cofactor Ca2+, buffer imidazole Botrytis cinerea
9.5
-
oxaloacetate cofactor Mg2+, buffer Tris Botrytis cinerea
9.6
-
oxaloacetate cofactor Mg2+, buffer Hepes Botrytis cinerea
10.3
-
oxaloacetate cofactor Mg2+, buffer imidazole Botrytis cinerea
11.8
-
oxaloacetate cofactor Mg2+, buffer imidazole Botrytis cinerea
12.5
-
oxaloacetate cofactor Mg2+, buffer Hepes Botrytis cinerea
32
-
oxaloacetate cofactor Mn2+, buffer Hepes Botrytis cinerea
37
-
oxaloacetate cofactor Mn2+, buffer imidazole Botrytis cinerea
50
-
oxaloacetate cofactor Mn2+, buffer imidazole Botrytis cinerea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
oxaloacetate hydrolase assay Botrytis cinerea

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000068
-
3,3-difluorooxaloacetate
-
Botrytis cinerea