Application | Comment | Organism |
---|---|---|
food industry | oxalate toxicity is a concern for the commercial application of fungi in the food and drug industries | Botrytis cinerea |
food industry | oxalate toxicity is a concern for the commercial application of fungi in the food and drug industries | Aspergillus niger |
Cloned (Comment) | Organism |
---|---|
into the vector pGEM-T Easy for sequencing | Aspergillus niger |
into the vector pGEM-T Easy for sequencing, and into pET-3c for expression in Escherichia coli BL21DE3 cells | Botrytis cinerea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,3-difluorooxaloacetate | - |
Aspergillus niger | |
3,3-difluorooxaloacetate | - |
Botrytis cinerea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.062 | - |
oxaloacetate | cofactor Mg2+, buffer imidazole | Botrytis cinerea | |
0.064 | - |
oxaloacetate | cofactor Mg2+, buffer Hepes | Botrytis cinerea | |
0.084 | - |
oxaloacetate | cofactor Mg2+, buffer Tris | Botrytis cinerea | |
0.24 | - |
oxaloacetate | cofactor Mn2+, buffer imidazole | Botrytis cinerea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34360 | - |
theoretical molecular mass | Botrytis cinerea |
35000 | - |
determined by SDS-PAGE | Botrytis cinerea |
100000 | - |
determined by gel filtration, indicative of a homotrimeric quaternary structure | Botrytis cinerea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + H2O | Botrytis cinerea | - |
oxalate + acetate | - |
? | |
oxaloacetate + H2O | Aspergillus niger | - |
oxalate + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | Q7Z986 | - |
- |
Botrytis cinerea | Q6PNM8 | - |
- |
Purification (Comment) | Organism |
---|---|
by ammonium sulfate-induced protein precipitation, and by using of a DEAE-cellulose and a butyl-Sepharose column | Botrytis cinerea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.62 | - |
cell extract | Botrytis cinerea |
4.6 | - |
purification step ammonium sulfate precipitation | Botrytis cinerea |
15 | - |
purification step DEAE-cellulose column | Botrytis cinerea |
20 | - |
purification step butyl-Sepharose column | Botrytis cinerea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + H2O | - |
Botrytis cinerea | oxalate + acetate | - |
? | |
oxaloacetate + H2O | - |
Aspergillus niger | oxalate + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the results are consistent with the existence of a monomer-dimer-tetramer equilibrium | Botrytis cinerea |
Synonyms | Comment | Organism |
---|---|---|
OAH | - |
Botrytis cinerea |
OAH | - |
Aspergillus niger |
oxaloacetate acetylhydrolase | - |
Botrytis cinerea |
oxaloacetate acetylhydrolase | - |
Aspergillus niger |
oxaloacetate hydrolase | - |
Botrytis cinerea |
oxaloacetate hydrolase | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
oxaloacetate hydrolase assay | Botrytis cinerea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
oxaloacetate | cofactor Ca2+, buffer imidazole | Botrytis cinerea | |
9.5 | - |
oxaloacetate | cofactor Mg2+, buffer Tris | Botrytis cinerea | |
9.6 | - |
oxaloacetate | cofactor Mg2+, buffer Hepes | Botrytis cinerea | |
10.3 | - |
oxaloacetate | cofactor Mg2+, buffer imidazole | Botrytis cinerea | |
11.8 | - |
oxaloacetate | cofactor Mg2+, buffer imidazole | Botrytis cinerea | |
12.5 | - |
oxaloacetate | cofactor Mg2+, buffer Hepes | Botrytis cinerea | |
32 | - |
oxaloacetate | cofactor Mn2+, buffer Hepes | Botrytis cinerea | |
37 | - |
oxaloacetate | cofactor Mn2+, buffer imidazole | Botrytis cinerea | |
50 | - |
oxaloacetate | cofactor Mn2+, buffer imidazole | Botrytis cinerea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
oxaloacetate hydrolase assay | Botrytis cinerea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000068 | - |
3,3-difluorooxaloacetate | - |
Botrytis cinerea |