Cloned (Comment) | Organism |
---|---|
cloned by inverse PCR into Escherichia coli | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
Mn2+ | - |
Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
10-12 * 39000, SDS-PAGE | Aspergillus niger |
360000 | 440000 | two major bands, native PAGE | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + H2O | Aspergillus niger | could be involved in pathogenesis and keeping pH low in vicinity of the fungus | oxalate + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
inducible enzyme, expressed only at pH values higher than 4.0 | - |
Purification (Comment) | Organism |
---|---|
Phenyl Sepharose, Q-Sepharose | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
56 | - |
- |
Aspergillus niger |
Storage Stability | Organism |
---|---|
4°C, 50 mM Tris-HCL, pH 7.5, 2 mM MnCl2, 20 mM DTT 5% sucrose, 7 days | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + H2O | could be involved in pathogenesis and keeping pH low in vicinity of the fungus | Aspergillus niger | oxalate + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | 10-12 * 39000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Aspergillus niger |