Literature summary for 3.6.5.5 extracted from

Chen, X.; Xu, X.; Sun, Y.; Zhou, J.; Ma, Y.; Yan, L.; Lou, Z.
Purification, crystallization and preliminary X-ray crystallographic analysis of Arabidopsis thaliana dynamin-related protein 1A GTPase-GED fusion protein (2012), Acta Crystallogr. Sect. F, 68, 69-72.

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of the GST-tagged chimeric fusion protein of AtDRP1A fusion protein in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha	Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant GST-tagged chimeric fusion protein of dynamin-related protein 1A, AtDRP1A, comprising GTPase domain of Arabidopsis thaliana AtDRP1A fused to its GTPase effector domain (GED) in a nucleotide-associated form, hanging drop vapour diffusion method, mixing of 5 mg/ml dimeric protein in 20 mM Tris-HCl, pH 8.0, 150 mM NaC, with 160 mM sodium formate, 16% w/v PEG 3350, 40 mM magnesium chloride, 6% w/v 2-propanol, 20 mM HEPES, pH 7.5, method optimization, X-ray diffraction structure determination and analysis at 3.6 A resolution, molecular replacement using the crystal structure of human dynamin GG dimer, PDB ID 2X2E	Arabidopsis thaliana

Crystallization (Comment)

Organism

purified recombinant GST-tagged chimeric fusion protein of dynamin-related protein 1A, AtDRP1A, comprising GTPase domain of Arabidopsis thaliana AtDRP1A fused to its GTPase effector domain (GED) in a nucleotide-associated form, hanging drop vapour diffusion method, mixing of 5 mg/ml dimeric protein in 20 mM Tris-HCl, pH 8.0, 150 mM NaC, with 160 mM sodium formate, 16% w/v PEG 3350, 40 mM magnesium chloride, 6% w/v 2-propanol, 20 mM HEPES, pH 7.5, method optimization, X-ray diffraction structure determination and analysis at 3.6 A resolution, molecular replacement using the crystal structure of human dynamin GG dimer, PDB ID 2X2E

Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of an AtDRP1A fusion protein consisting of residues 1-316 of the GTPase domain and residues 585-606 of GED fused by a flexible linker with the primary sequence HGTDSRV as suggested from human dynamin	Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39000	-	dimer of dimers, 4 * 39000, crystal structure analysis	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GTP + H2O	Arabidopsis thaliana	-	GDP + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	P42697	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant GST-tagged chimeric fusion protein of AtDRP1A fusion protein from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + H2O	-	Arabidopsis thaliana	GDP + phosphate	-	?

Subunits

Subunits	Comment	Organism
tetramer	dimer of dimers, 4 * 39000, crystal structure analysis	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
AtDRP1A	-	Arabidopsis thaliana
dynamin-related protein 1A	-	Arabidopsis thaliana

General Information

General Information	Comment	Organism
evolution	enzyme AtDRP1A belongss to the DRP1 dynamin-related subfamily of the superfamily of GTPases. Dynamins and other structurally related family members primarily consist of a GTPase domain, a middle domain and a GTPase effector domain, which regulates and responds to hydrolysis of the GTPase domain. The GED and the Nand C-termini of the GTPase domain form a so-called three-helical bundle signalling element that regulates dynamin function	Arabidopsis thaliana
metabolism	plant-specific dynamin-related proteins play crucial roles in cell-plate formation, endocytosis or exocytosis, protein sorting to the vacuole and plasma membrane and the division of mitochondria and chloroplasts	Arabidopsis thaliana
physiological function	interaction between the enzyme's GTPase domain and its GED domain is important for regulating dynamin function	Arabidopsis thaliana