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Literature summary for 3.6.5.5 extracted from
- Characterization of PfDYN2, a dynamin-like protein of Plasmodium falciparum expressed in schizonts (2007), Microbes Infect., 9, 797-805.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the pT-Adv vector, into pET-15b for expression in Escherichia coli BL21DE3codon-plus cells, a 711 bp fragment corresponding to the GTPase N-terminal domain into pET-22b, and into the pSynXIV VI+X3 vector for recombinat expression in the BEV system | Plasmodium falciparum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| apicoplast | - |
Plasmodium falciparum | 20011 | - |
| cytoplasm | - |
Plasmodium falciparum | 5737 | - |
| endoplasmic reticulum | - |
Plasmodium falciparum | 5783 | - |
| Golgi apparatus | - |
Plasmodium falciparum | 5794 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 83000 | - |
determind by SDS-PAGE and Western-blotting | Plasmodium falciparum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | Plasmodium falciparum | - |
GDP + phosphate | - |
ir |  |
| GTP + H2O | Plasmodium falciparum FcB1 | - |
GDP + phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | Q8IJ32 | - |
- |
| Plasmodium falciparum FcB1 | Q8IJ32 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a nickel-agarose column | Plasmodium falciparum |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| the DYN2 N-terminal and the full-length protein, expressed in BL21DE3codon-plus cells, are purified from inclusion bodies under denaturing conditions | Plasmodium falciparum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| schizont | - |
Plasmodium falciparum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | - |
Plasmodium falciparum | GDP + phosphate | - |
ir | |
| GTP + H2O | - |
Plasmodium falciparum FcB1 | GDP + phosphate | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DYN2 | - |
Plasmodium falciparum |
| dynamin GTPase | - |
Plasmodium falciparum |
| dynamin2 | - |
Plasmodium falciparum |
| PfDYN2 | - |
Plasmodium falciparum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
GTPase assay at room temperature | Plasmodium falciparum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
GTPase assay | Plasmodium falciparum |
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