Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli B834(DE3)-Rosetta2 cells | Pyrococcus furiosus |
Crystallization (Comment) | Organism |
---|---|
GDP-bound subunit SRP54 and free subunit SRP19 are crystallized by hanging drop vapour diffusion method, crystals of SRP54 grow in 1.0-1.3 M lithium sulfate and 100 mM sodium acetate pH 5.0, crystals of SRP19 grow in 1.2-1.3 M sodium malonate and 100 mM sodium acetate pH 5.0 | Pyrococcus furiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U070 | - |
- |
Purification (Comment) | Organism |
---|---|
heat selective precipitation, cobalt-chelating affinity chromatography, gel filtration, and ion-exchange chromatography | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Pyrococcus furiosus | GDP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | subunit SRP54, X-ray crystallography | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
Ffh | - |
Pyrococcus furiosus |
signal recognition particle | - |
Pyrococcus furiosus |
SRP | - |
Pyrococcus furiosus |
SRP19 | accessory subunit of the signal recognition particle, SRP54 and SRP19 are the two RNA binding components forming the core of the signal recognition particle, SRP19 acts as a molecular scaffold and a chaperone, assisting the SRP RNA in adopting the conformation required for its optimal interaction with the essential subunit SRP54, and proper assembly of a functional SRP | Pyrococcus furiosus |
SRP54 | GTPase subunit of the signal recognition particle, SRP54 and SRP19 are the two RNA binding components forming the core of the signal recognition particle | Pyrococcus furiosus |