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Literature summary for 3.6.5.4 extracted from

  • Egea, P.F.; Napetschnig, J.; Walter, P.; Stroud, R.M.
    Structures of SRP54 and SRP19, the two proteins that organize the ribonucleic core of the signal recognition particle from Pyrococcus furiosus (2008), PLoS ONE, 3, e3528.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli B834(DE3)-Rosetta2 cells Pyrococcus furiosus

Crystallization (Commentary)

Crystallization (Comment) Organism
GDP-bound subunit SRP54 and free subunit SRP19 are crystallized by hanging drop vapour diffusion method, crystals of SRP54 grow in 1.0-1.3 M lithium sulfate and 100 mM sodium acetate pH 5.0, crystals of SRP19 grow in 1.2-1.3 M sodium malonate and 100 mM sodium acetate pH 5.0 Pyrococcus furiosus

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U070
-
-

Purification (Commentary)

Purification (Comment) Organism
heat selective precipitation, cobalt-chelating affinity chromatography, gel filtration, and ion-exchange chromatography Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
Pyrococcus furiosus GDP + phosphate
-
?

Subunits

Subunits Comment Organism
dimer subunit SRP54, X-ray crystallography Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
Ffh
-
Pyrococcus furiosus
signal recognition particle
-
Pyrococcus furiosus
SRP
-
Pyrococcus furiosus
SRP19 accessory subunit of the signal recognition particle, SRP54 and SRP19 are the two RNA binding components forming the core of the signal recognition particle, SRP19 acts as a molecular scaffold and a chaperone, assisting the SRP RNA in adopting the conformation required for its optimal interaction with the essential subunit SRP54, and proper assembly of a functional SRP Pyrococcus furiosus
SRP54 GTPase subunit of the signal recognition particle, SRP54 and SRP19 are the two RNA binding components forming the core of the signal recognition particle Pyrococcus furiosus