Cloned (Comment) | Organism |
---|---|
the NG domain of Ffh is cloned into pUCm-T, and into pET-15b for expression in Escherichia coli BL21 cells | Streptomyces coelicolor |
the NG domain of FtsY is cloned into pUCm-T, and into pET-15b for expression in Escherichia coli BL21 cells | Streptomyces coelicolor |
Protein Variants | Comment | Organism |
---|---|---|
A145S | substitution introduced into the putative GTP binding motif GXXGXGK | Streptomyces coelicolor |
G141V | substitution introduced into the putative GTP binding motif GXXGXGK | Streptomyces coelicolor |
G222V | substitution introduced into the putative GTP binding motif GXXGXGK | Streptomyces coelicolor |
GAK-VSG | substitution introduced into the putative GTP binding motif GXXGXGK | Streptomyces coelicolor |
GTK-VSG | substitution introduced into the putative GTP binding motif GXXGXGK | Streptomyces coelicolor |
K147G | substitution introduced into the putative GTP binding motif GXXGXGK | Streptomyces coelicolor |
K228G | substitution introduced into the putative GTP binding motif GXXGXGK | Streptomyces coelicolor |
T226S | substitution introduced into the putative GTP binding motif GXXGXGK | Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00081 | - |
GTP | NG-Ffh, wild-type | Streptomyces coelicolor | |
0.001131 | - |
GTP | NG-Ffh, mutant A145S | Streptomyces coelicolor | |
0.001143 | - |
GTP | NG-FtsY, wild-type | Streptomyces coelicolor | |
0.001183 | - |
GTP | NG-FtsY, mutant T226S | Streptomyces coelicolor | |
0.001265 | - |
GTP | NG-Ffh, mutant G141V | Streptomyces coelicolor | |
0.002879 | - |
GTP | NG-FtsY, mutant K228G | Streptomyces coelicolor | |
0.00349 | - |
GTP | NG-FtsY, mutant GTK-VSG | Streptomyces coelicolor | |
0.00493 | - |
GTP | NG-FtsY, mutant G222V | Streptomyces coelicolor | |
0.005075 | - |
GTP | NG-Ffh, mutant K147G | Streptomyces coelicolor | |
0.005128 | - |
GTP | NG-Ffh, mutant GAK-VSG | Streptomyces coelicolor |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Streptomyces coelicolor | 5737 | - |
membrane | - |
Streptomyces coelicolor | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
NG domain of Ffh, determined by SDS-PAGE | Streptomyces coelicolor |
36000 | - |
NG domain of FtsY, determined by SDS-PAGE | Streptomyces coelicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Streptomyces coelicolor | - |
GDP + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
- |
- |
Purification (Comment) | Organism |
---|---|
proteins are purified using histidine-binding magnetic agarose beads | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Streptomyces coelicolor | GDP + phosphate | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
Ffh | - |
Streptomyces coelicolor |
FtsY | - |
Streptomyces coelicolor |
signal-recognition-particle GTPase | - |
Streptomyces coelicolor |
SRP GTPase | - |
Streptomyces coelicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
GTPase activity assay | Streptomyces coelicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
GTPase activity assay | Streptomyces coelicolor |