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Literature summary for 3.6.5.3 extracted from

  • De Vendittis, E.; Adinolfi, B.S.; Amatruda, M.R.; Raimo, G.; Masullo, M.; Bocchini, V.
    The A26G replacement in the consensus sequence A-X-X-X-X-G-K-[T,S] of the guanine nucleotide binding site activates the intrinsic GTPase of the elongation factor 2 from the archaeon Sulfolobus solfataricus (1999), Eur. J. Biochem., 262, 600-605.
    View publication on PubMed
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Protein Variants

Protein Variants Comment Organism
A26G converts the guanine nucleotide binding consensus sequences A-X-X-X-X-G-K-[T,S] of the elongation factor EF-2 into the corresponding G-X-X-X-X-G-K-[T,S] motif which is present in all the other GTP-binding proteins. In the mutant, the rate of poly(U)-directed poly(Phe) synthesis and the ribosome-dependent GTPase activity of A26GSsEF-2 are decreased. A26G substitution enhances the catalytic efficiency of the intrinsic SsEF-2 GTPase triggered by ethylene glycol and decreases the affinity for GDP Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
GDP
-
 Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
 GTP mutant A26G, pH 7.5, 60°C, presence of 40% ethylene glycol Saccharolobus solfataricus
0.001
-
 GTP wild-type, pH 7.5, 60°C, presence of 40% ethylene glycol Saccharolobus solfataricus
0.0055
-
 GTP mutant A26G, pH 7.5, 60°C, presence of 10% ethylene glycol Saccharolobus solfataricus
0.0096
-
 GTP wild-type, pH 7.5, 60°C, presence of 10% ethylene glycol Saccharolobus solfataricus
0.0194
-
 GTP mutant A26G, pH 7.5, 60°C Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Saccharolobus solfataricus GDP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
elongation factor 2
-
 Saccharolobus solfataricus
SsEF-2
-
 Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0033
-
 GTP wild-type, pH 7.5, 60°C, presence of 10% ethylene glycol Saccharolobus solfataricus
0.032
-
 GTP wild-type, pH 7.5, 60°C, presence of 40% ethylene glycol Saccharolobus solfataricus
0.12
-
 GTP mutant A26G, pH 7.5, 60°C, presence of 10% ethylene glycol Saccharolobus solfataricus
0.133
-
 GTP mutant A26G, pH 7.5, 60°C Saccharolobus solfataricus
0.165
-
 GTP mutant A26G, pH 7.5, 60°C, presence of 40% ethylene glycol Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0003
-
 GDP wild-type, pH 7.5, 60°C, presence of 40% ethylene glycol Saccharolobus solfataricus
0.0005
-
 GDP mutant A26G, pH 7.5, 60°C, presence of 40% ethylene glycol Saccharolobus solfataricus
0.0074
-
 GDP mutant A26G, pH 7.5, 60°C Saccharolobus solfataricus