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Literature summary for 3.6.5.2 extracted from

  • Moon, M.Y.; Kim, H.J.; Kim, J.G.; Lee, J.Y.; Kim, J.; Kim, S.C.; Choi, I.G.; Kim, P.H.; Park, J.B.
    Small GTPase Rap1 regulates cell migration through regulation of small GTPase RhoA activity in response to transforming growth factor-beta1 (2013), J. Cell. Physiol., 228, 2119-2126.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
TGF-beta1 TGF-beta1 activates Rap1 through cAMP and Epac1/Epac2. 2',5-dideoxyadenosine is an inhibitor of adenlyate cyclase on Rap1 activity Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of GST-tagged RapI protein Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Mus musculus
-
GDP + phosphate
-
?
GTP + H2O Mus musculus C57BL/6
-
GDP + phosphate
-
?
additional information Mus musculus Ras-related, small GTPases act as molecular switches that control a variety of cellular processes by cycling between alternative conformational states: in the active state, they are bound with GTP, and in the inactive state, they are bound with GDP. In their active state, GTPases recognize their target effector proteins and evoke responses until GTP hydrolysis returns the switch to the off position ?
-
?
additional information Mus musculus C57BL/6 Ras-related, small GTPases act as molecular switches that control a variety of cellular processes by cycling between alternative conformational states: in the active state, they are bound with GTP, and in the inactive state, they are bound with GDP. In their active state, GTPases recognize their target effector proteins and evoke responses until GTP hydrolysis returns the switch to the off position ?
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus Q91VL8
-
-
Mus musculus Q9QUI0
-
-
Mus musculus C57BL/6 Q91VL8
-
-
Mus musculus C57BL/6 Q9QUI0
-
-

Source Tissue

Source Tissue Comment Organism Textmining
macrophage
-
Mus musculus
-
RAW-264.7 cell
-
Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
Mus musculus GDP + phosphate
-
?
GTP + H2O
-
Mus musculus C57BL/6 GDP + phosphate
-
?
additional information Ras-related, small GTPases act as molecular switches that control a variety of cellular processes by cycling between alternative conformational states: in the active state, they are bound with GTP, and in the inactive state, they are bound with GDP. In their active state, GTPases recognize their target effector proteins and evoke responses until GTP hydrolysis returns the switch to the off position Mus musculus ?
-
?
additional information Ras-related, small GTPases act as molecular switches that control a variety of cellular processes by cycling between alternative conformational states: in the active state, they are bound with GTP, and in the inactive state, they are bound with GDP. In their active state, GTPases recognize their target effector proteins and evoke responses until GTP hydrolysis returns the switch to the off position Mus musculus C57BL/6 ?
-
?

Synonyms

Synonyms Comment Organism
Rap1
-
Mus musculus
RhoA
-
Mus musculus
small GTPase
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Mus musculus

Expression

Organism Comment Expression
Mus musculus 8CPT-2Me-cAMP suppresses GTP-RhoA levels down
Mus musculus si-RNAs of Epac1 and Epac2 reduced GTP-Rap1 levels down
Mus musculus si-Rap1 augments GTP-RhoA levels and cell migration. si-RNAs of Epac1 and Epac2 reduced GTP-Rap1 levels leading to promotion of GTP-RhoA levels. si-RNA of ARAP3 (Rap-dependent RhoGAP) increased GTP-RhoA level and cell migration up

General Information

General Information Comment Organism
physiological function small GTPase Rap1 regulates cell migration through regulation of small GTPase RhoA activity in response to transforming growth factor-beta1. GTP-RhoA and GTP-Rap1 levels are reciprocally regulated in a time-dependent manner following TGF-beta1 stimulation. Ras-related, small GTPases act as molecular switches that control a variety of cellular processes by cycling between alternative conformational states. In the active state, they are bound with GTP, and in the inactive state, they are bound with GDP. In their active state, GTPases recognize their target effector proteins and evoke responses until GTP hydrolysis returns the switch to the off position. GTP-binding to small GTPases is catalyzed by guanine nucleotide exchange factors (GEFs), and hydrolysis of the bound GTP is accelerated by GTPase activating proteins (GAPs), Downstream effector proteins of Rap1, including RapL, AF6, and ARAP3 transmit the activated Rap1 signals within the cells. Rap1 regulates GTPase RhoA activity, but RhoA does not regulate the Rap1 activity Mus musculus
physiological function small GTPase Rap1 regulates cell migration through regulation of small GTPase RhoA activity in response to transforming growth factor-beta1. GTP-RhoA and GTP-Rap1 levels are reciprocally regulated in a time-dependent manner following TGF-beta1 stimulation. Ras-related, small GTPases act as molecular switches that control a variety of cellular processes by cycling between alternative conformational states. In the active state, they are bound with GTP, and in the inactive state, they are bound with GDP. In their active state, GTPases recognize their target effector proteins and evoke responses until GTP hydrolysis returns the switch to the off position. GTP-binding to small GTPases is catalyzed by guanine nucleotide exchange factors (GEFs), and hydrolysis of the bound GTP is accelerated by GTPase activating proteins (GAPs). Downstream effector proteins of Rap1, including RapL, AF6, and ARAP3 transmit the activated Rap1 signals within the cells. Rap1 regulates GTPase RhoA activity, but RhoA does not regulate the Rap1 activity Mus musculus