Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.5.1 extracted from

  • Romero-Castillo, R.A.; Roy Choudhury, S.; Leon-Felix, J.; Pandey, S.
    Characterization of the heterotrimeric G-protein family and its transmembrane regulator from capsicum (Capsicum annuum L.) (2015), Plant Sci., 234, 97-109.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
RGS protein one RGS gene in the Capsicum genome that acts as a regulator of the G-protein signaling, idetification and cloning Capsicum annuum

Cloned(Commentary)

Cloned (Comment) Organism
cloning of the complete heterotrimeric G-protein repertoire in the Capsicum annuum genome which consists of one Galpha, one Gbeta and three Ggamma genes, phylogenetic analysis, DNA and amino acid sequence determination and analysis, real time quantitative PCR tissue-specific enzyme expression analysis, transgenic expression of Gbeta, Ggamma1-3 genes, from pSAT5-DEST-cEYFP-C1 destination vectors containing cEYFP at the N-terminal end via Agrobacterium tumefaciens strain GV3101 transformation, in Nicotiana tabaccum leaves, co-expression of RGS protein Capsicum annuum

Protein Variants

Protein Variants Comment Organism
additional information protein CaG alpha1 can complement the Galpha-deficient Saccharomyces cerevisiae Gpa1 mutant YGS5 Capsicum annuum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Capsicum annuum
-
GDP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Capsicum annuum
-
cv. California wonder
-

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Capsicum annuum
-
flower
-
Capsicum annuum
-
flower bud
-
Capsicum annuum
-
fruit
-
Capsicum annuum
-
leaf
-
Capsicum annuum
-
additional information expression pattern of each of the G-protein genes, ofCaGalpha1, CaGbeta1, CaGgamma1-3 and of CaRGS1 in different tissue types, overview Capsicum annuum
-
root
-
Capsicum annuum
-
seed
-
Capsicum annuum
-
seedling
-
Capsicum annuum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
Capsicum annuum GDP + phosphate
-
?
GTP + H2O specific GTP/GDP-binding and GTP-hydrolysis activity of recombinant CaGalpha1 protein, no binding of ATP or ADP Capsicum annuum GDP + phosphate
-
?
additional information analysis of interaction between Capsicum Galpha and Gbeta proteins and between Galpha and RGS activator proteins by yeast split-ubiquitin system Capsicum annuum ?
-
?

General Information

General Information Comment Organism
additional information the complete heterotrimeric G-protein repertoire in the Capsicum annuum genome consists of one Galpha, one Gbeta and three Ggamma genes Capsicum annuum
physiological function in plants, heterotrimeric G-protein complexes play important roles in signal transduction pathways related to growth and development including response to biotic and abiotic stresses and consequently affect yield of crop plants Capsicum annuum