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Literature summary for 3.6.5.1 extracted from
- The Ras-binding domain region of RGS14 regulates its functional interactions with heterotrimeric G proteins (2013), J. Cell. Biochem., 114, 1414-1423.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| RGS14 protein | serves as a GTPase accelerating protein for receptor-coupled heterotrimeric G proteins. RGS14 is a 60 kDa protein of the D/R12 subfamily that contains a regulator of G protein signalling (RGS) domain near its N-terminus, a central region containing a pair of tandem Ras binding domains (RBD), and a GPSM (G protein signalling modulator) domain near its C-terminus. The RGS domain of RGS14 exhibits GTPase accelerating protein activity toward Galphai/o proteins, while its GPSM domain acts as a guanine nucleotide dissociation inhibitor on Galphai1 and Galphai3. The full-length protein has a greater GTPase activating activity but a weaker inhibition of nucleotide dissociation relative to its isolated RGS and GPSM regions, respectively. These differences may be attributable to an inter-domain interaction within RGS14 that promotes the activity of the RGS domain, but simultaneously inhibits the activity of the GPSM domain. The RBD region seems to play an essential role in this regulatory activity. Various potential alternative splice variants of RGS14 are identified. Purified R14-RBD alone has no appreciable effect on the GTPase activity of M2 muscarinic receptor-stimulated Galphai3. The GPSM domain of RGS14 does not inhibit receptor-stimulated GTPase activity | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of heterotrimeric G-proteins Galphai1 and Galphai3 in Spodoptera frugiperda Sf 9 cell membranes using the baculovirus transfection method | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | Homo sapiens | - |
GDP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | - |
Homo sapiens | GDP + phosphate | - |
? | |
| additional information | activator/regulator protein R14-RGS by itself shows only a marginal ability to promote GTPase activity | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Galphai1 | - |
Homo sapiens |
| Galphai3 | - |
Homo sapiens |
| heterotrimeric G protein | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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