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Literature summary for 3.6.5.1 extracted from

  • Won, S.; Michkov, A.V.; Krystofova, S.; Garud, A.V.; Borkovich, K.A.
    Genetic and physical interactions between Galpha subunits and components of the Gbetagamma dimer of heterotrimeric G proteins in Neurospora crassa (2012), Eukaryot. Cell, 11, 1239-1248.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
genes gna 1-3 encoding the three subunits of the enzyme, transfection of Neurospora crassa with mutant enzymes Neurospora crassa

Protein Variants

Protein Variants Comment Organism
additional information construction of several subunit deletion mutants, creation of double mutants lacking one Galpha gene and gnb-1 and introduced constitutively active, GTPase-deficient alleles for each Galpha gene into the DELTAgnb-1 background, phenotypes. Genetic analysis reveals that gna-3 is epistatic to gnb-1 with regard to negative control of submerged conidiation. gnb-1 is epistatic to gna-2 and gna-3 for aerial hyphal height, while gnb-1 appears to act upstream of gna-1 and gna-2 during aerial conidiation. None of the activated Galpha alleles restore female fertility to DELTAgnb-1 mutants, and the gna-3Q208L allele inhibits formation of female reproductive structures, consistent with a need for Galpha proteins to cycle through the inactive GDP-bound form for these processes. Introduction of activated Galpha alleles into the DELTAgnb-1 background restores Galpha protein levels Neurospora crassa
Q204L site-directed mutaggenesis Neurospora crassa
Q205L site-directed mutaggenesis Neurospora crassa
Q208L site-directed mutaggenesis Neurospora crassa
R179C site-directed mutaggenesis Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa Q05425 and Q05424 and Q9HFW7 genes gna 1-3 encoding the three subunits of the enzyme
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Neurospora crassa 74 A-OR23-1A Q05425 and Q05424 and Q9HFW7 genes gna 1-3 encoding the three subunits of the enzyme
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Subunits

Subunits Comment Organism
heteropentamer (alpha)3(betagamma), three Galpha subunits (GNA-1, GNA-2, and GNA-3), one Gbeta subunit (GNB-1), and one Ggamma subunit (GNG-1), the Gbetagamma dimer interacts with all three Galpha proteins, epistasis between gnb-1 and gna-1, gna-2, and gna-3 for at least one function Neurospora crassa

General Information

General Information Comment Organism
malfunction none of the activated Galpha alleles restore female fertility to DELTAgnb-1 mutants, and the gna-3Q208L allele inhibits formation of female reproductive structures, consistent with a need for Galpha proteins to cycle through the inactive GDP-bound form for these processes. During the sexual cycle, DELTAgnb-1 and DELTAgna-1 mutants are male fertile but female sterile, Although these strains produce protoperithecia and trichogynes, their trichogynes have a defect in chemotropism and are not attracted by male cells. DELTAgna-2 and DELTAgna-3 mutants produce protoperithecia and develop perithecia after fertilization with wild-type males. Mutant phenotypes during asexual growth and development, overview Neurospora crassa
additional information relationship between the three Galpha subunits and components of the Gbetagamma dimer, physical interaction between the Gbeta and Ggamma subunits, overview Neurospora crassa
physiological function heterotrimeric G proteins are critical regulators of growth and asexual and sexual development in the filamentous fungus Neurospora crassa, wild-type phenotype during asexual growth and development Neurospora crassa