Cloned (Comment) | Organism |
---|---|
genes gna 1-3 encoding the three subunits of the enzyme, transfection of Neurospora crassa with mutant enzymes | Neurospora crassa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of several subunit deletion mutants, creation of double mutants lacking one Galpha gene and gnb-1 and introduced constitutively active, GTPase-deficient alleles for each Galpha gene into the DELTAgnb-1 background, phenotypes. Genetic analysis reveals that gna-3 is epistatic to gnb-1 with regard to negative control of submerged conidiation. gnb-1 is epistatic to gna-2 and gna-3 for aerial hyphal height, while gnb-1 appears to act upstream of gna-1 and gna-2 during aerial conidiation. None of the activated Galpha alleles restore female fertility to DELTAgnb-1 mutants, and the gna-3Q208L allele inhibits formation of female reproductive structures, consistent with a need for Galpha proteins to cycle through the inactive GDP-bound form for these processes. Introduction of activated Galpha alleles into the DELTAgnb-1 background restores Galpha protein levels | Neurospora crassa |
Q204L | site-directed mutaggenesis | Neurospora crassa |
Q205L | site-directed mutaggenesis | Neurospora crassa |
Q208L | site-directed mutaggenesis | Neurospora crassa |
R179C | site-directed mutaggenesis | Neurospora crassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | Q05425 and Q05424 and Q9HFW7 | genes gna 1-3 encoding the three subunits of the enzyme | - |
Neurospora crassa 74 A-OR23-1A | Q05425 and Q05424 and Q9HFW7 | genes gna 1-3 encoding the three subunits of the enzyme | - |
Subunits | Comment | Organism |
---|---|---|
heteropentamer | (alpha)3(betagamma), three Galpha subunits (GNA-1, GNA-2, and GNA-3), one Gbeta subunit (GNB-1), and one Ggamma subunit (GNG-1), the Gbetagamma dimer interacts with all three Galpha proteins, epistasis between gnb-1 and gna-1, gna-2, and gna-3 for at least one function | Neurospora crassa |
General Information | Comment | Organism |
---|---|---|
malfunction | none of the activated Galpha alleles restore female fertility to DELTAgnb-1 mutants, and the gna-3Q208L allele inhibits formation of female reproductive structures, consistent with a need for Galpha proteins to cycle through the inactive GDP-bound form for these processes. During the sexual cycle, DELTAgnb-1 and DELTAgna-1 mutants are male fertile but female sterile, Although these strains produce protoperithecia and trichogynes, their trichogynes have a defect in chemotropism and are not attracted by male cells. DELTAgna-2 and DELTAgna-3 mutants produce protoperithecia and develop perithecia after fertilization with wild-type males. Mutant phenotypes during asexual growth and development, overview | Neurospora crassa |
additional information | relationship between the three Galpha subunits and components of the Gbetagamma dimer, physical interaction between the Gbeta and Ggamma subunits, overview | Neurospora crassa |
physiological function | heterotrimeric G proteins are critical regulators of growth and asexual and sexual development in the filamentous fungus Neurospora crassa, wild-type phenotype during asexual growth and development | Neurospora crassa |