Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | GTPase activity is stimulated by ribosomes up to 2000fold | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP | competitive inhibition | Saccharolobus solfataricus | |
Gpp(NH)p | competitive inhibition | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
GTP | 50°C | Saccharolobus solfataricus | |
0.009 | - |
GTP | 60°C | Saccharolobus solfataricus | |
0.01 | - |
GTP | 70°C | Saccharolobus solfataricus | |
0.011 | - |
GTP | 75°C | Saccharolobus solfataricus | |
0.013 | - |
GTP | 80°C | Saccharolobus solfataricus | |
0.032 | - |
GTP | 87°C | Saccharolobus solfataricus | |
0.148 | - |
GTP | 91°C | Saccharolobus solfataricus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
ribosome | - |
Saccharolobus solfataricus | 5840 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Saccharolobus solfataricus | - |
GDP + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified from Sulfolobus solfataricus | Saccharolobus solfataricus |
Storage Stability | Organism |
---|---|
-20°C, Tris-HCl buffer, pH 7.8, 10 mM MgCl2, 50% v/v glycerol | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Saccharolobus solfataricus | GDP + phosphate | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
elongation factor 2 | - |
Saccharolobus solfataricus |
heterotrimeric G-protein GTPase | - |
Saccharolobus solfataricus |
SsEF-2 | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
GTP | 50°C | Saccharolobus solfataricus | |
0.8 | - |
GTP | 60°C | Saccharolobus solfataricus | |
1.7 | - |
GTP | 70°C | Saccharolobus solfataricus | |
2.7 | - |
GTP | 75°C | Saccharolobus solfataricus | |
3.5 | - |
GTP | 80°C | Saccharolobus solfataricus | |
6.2 | - |
GTP | 87°C | Saccharolobus solfataricus | |
8.8 | - |
GTP | 91°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Saccharolobus solfataricus |