Activating Compound | Comment | Organism | Structure |
---|---|---|---|
RGS4 | - |
Mammalia |
Cloned (Comment) | Organism |
---|---|
into vector for expression in Escherichia coli | Mammalia |
Crystallization (Comment) | Organism |
---|---|
crystal structure of mutant K180P-Galphai1 bound to a GTP analog | Mammalia |
Protein Variants | Comment | Organism |
---|---|---|
G202A | mutation of Galphai1 accelerates the rates of GTP hydrolysis and conformational change | Mammalia |
G202A, K180P | mutations of Galphai1 accelerate the rates of GTP hydrolysis | Mammalia |
K180A | mutant of Galphai1, rate of GTP hydrolysis similar to wild-type | Mammalia |
K180P | mutation of Galphai1 increases the rate of conformational change and decreases the rates of GTP hydrolysis | Mammalia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mammalia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Mammalia | - |
GDP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mammalia | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a Ni-NTA column | Mammalia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Mammalia | GDP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | Galpha subunit, GDP-bound inactive, GTP-bound active and betagamma dimer | Mammalia |
Synonyms | Comment | Organism |
---|---|---|
GTP phosphohydrolase | - |
Mammalia |
heterotrimeric G-protein GTPase | - |
Mammalia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
single-turnover GTPase assay | Mammalia |