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Literature summary for 3.6.5.1 extracted from

  • Nakamura, F.; Kato, M.; Kameyama, K.; Nukada, T.; Haga, T.; Kato, H.; Takenawa, T.; Kikkawa, U.
    Characterization of Gq family G proteins GL1alpha(G14alpha), GL2alpha(G11alpha), and Gqalpga expressed in the baculovirus-insect cell system (1995), J. Biol. Chem., 270, 6246-6253.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
muscarinic acetylcholine receptors receptor m1 activates, no activation by receptor m2 Bos taurus

Cloned(Commentary)

Cloned (Comment) Organism
the alpha subunit of Gq family G proteins, GL1alpha(G14alpha), GL2alpha(G11alpha) and Gqalpha are expressed with G proteins beta1 and gamma2 subunits in insect cells using a baculovirus system. The trimeric forms of G proteins, GL1(GL1alphabetagamma), GL2(GL2alphabetagamma) and Gq(Gqalphabetagamma) are solubilized and purified Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
8000
-
alpha,beta,gamma, 1 * 42000 + 1 * 36000 (beta1) + 1 * 8000 (gamma1), GL1 or Gq protein, SDS-PAGE Bos taurus
8000
-
alpha,beta,gamma, 1 * 43000 + 1 * 36000, (beta1) + 1 * 8000 (gamma2), GL1 protein, SDS-PAGE Bos taurus
36000
-
alpha,beta,gamma, 1 * 42000 + 1 * 36000 (beta1) + 1 * 8000 (gamma1), GL1 or Gq protein, SDS-PAGE Bos taurus
36000
-
alpha,beta,gamma, 1 * 43000 + 1 * 36000, (beta1) + 1 * 8000 (gamma2), GL1 protein, SDS-PAGE Bos taurus
36000
-
alpha,beta,gamma, 1 * 39000 + 1 * 36000 (beta1) + 1 * 80000 (gamma2), Go protein, SDS-PAGE Bos taurus
39000
-
alpha,beta,gamma, 1 * 39000 + 1 * 36000 (beta1) + 1 * 80000 (gamma2), Go protein, SDS-PAGE Bos taurus
42000
-
alpha,beta,gamma, 1 * 42000 + 1 * 36000 (beta1) + 1 * 8000 (gamma1), GL1 or Gq protein, SDS-PAGE Bos taurus
43000
-
alpha,beta,gamma, 1 * 43000 + 1 * 36000, (beta1) + 1 * 8000 (gamma2), GL1 protein, SDS-PAGE Bos taurus
80000
-
alpha,beta,gamma, 1 * 39000 + 1 * 36000 (beta1) + 1 * 80000 (gamma2), Go protein, SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
the alpha subunit of Gq family G proteins, GL1alpha(G14alpha), GL2alpha(G11alpha) and Gqalpha are expressed with G proteins beta1 and gamma2 subunits in insect cells using a baculovirus system. The trimeric forms of G proteins, GL1(GL1alphabetagamma), GL2(GL2alphabetagamma) and Gq(Gqalphabetagamma) are solubilized and purified Bos taurus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.722
-
recombinant GL2 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O activates phosphoinositide-specific phospholipase C in the presence of aluminium fluoride Bos taurus GDP + phosphate
-
?

Subunits

Subunits Comment Organism
? alpha,beta,gamma, 1 * 42000 + 1 * 36000 (beta1) + 1 * 8000 (gamma1), GL1 or Gq protein, SDS-PAGE Bos taurus
? alpha,beta,gamma, 1 * 43000 + 1 * 36000, (beta1) + 1 * 8000 (gamma2), GL1 protein, SDS-PAGE Bos taurus
? alpha,beta,gamma, 1 * 39000 + 1 * 36000 (beta1) + 1 * 80000 (gamma2), Go protein, SDS-PAGE Bos taurus