Activating Compound | Comment | Organism | Structure |
---|---|---|---|
muscarinic acetylcholine receptors | receptor m1 activates, no activation by receptor m2 | Bos taurus |
Cloned (Comment) | Organism |
---|---|
the alpha subunit of Gq family G proteins, GL1alpha(G14alpha), GL2alpha(G11alpha) and Gqalpha are expressed with G proteins beta1 and gamma2 subunits in insect cells using a baculovirus system. The trimeric forms of G proteins, GL1(GL1alphabetagamma), GL2(GL2alphabetagamma) and Gq(Gqalphabetagamma) are solubilized and purified | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
8000 | - |
alpha,beta,gamma, 1 * 42000 + 1 * 36000 (beta1) + 1 * 8000 (gamma1), GL1 or Gq protein, SDS-PAGE | Bos taurus |
8000 | - |
alpha,beta,gamma, 1 * 43000 + 1 * 36000, (beta1) + 1 * 8000 (gamma2), GL1 protein, SDS-PAGE | Bos taurus |
36000 | - |
alpha,beta,gamma, 1 * 42000 + 1 * 36000 (beta1) + 1 * 8000 (gamma1), GL1 or Gq protein, SDS-PAGE | Bos taurus |
36000 | - |
alpha,beta,gamma, 1 * 43000 + 1 * 36000, (beta1) + 1 * 8000 (gamma2), GL1 protein, SDS-PAGE | Bos taurus |
36000 | - |
alpha,beta,gamma, 1 * 39000 + 1 * 36000 (beta1) + 1 * 80000 (gamma2), Go protein, SDS-PAGE | Bos taurus |
39000 | - |
alpha,beta,gamma, 1 * 39000 + 1 * 36000 (beta1) + 1 * 80000 (gamma2), Go protein, SDS-PAGE | Bos taurus |
42000 | - |
alpha,beta,gamma, 1 * 42000 + 1 * 36000 (beta1) + 1 * 8000 (gamma1), GL1 or Gq protein, SDS-PAGE | Bos taurus |
43000 | - |
alpha,beta,gamma, 1 * 43000 + 1 * 36000, (beta1) + 1 * 8000 (gamma2), GL1 protein, SDS-PAGE | Bos taurus |
80000 | - |
alpha,beta,gamma, 1 * 39000 + 1 * 36000 (beta1) + 1 * 80000 (gamma2), Go protein, SDS-PAGE | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the alpha subunit of Gq family G proteins, GL1alpha(G14alpha), GL2alpha(G11alpha) and Gqalpha are expressed with G proteins beta1 and gamma2 subunits in insect cells using a baculovirus system. The trimeric forms of G proteins, GL1(GL1alphabetagamma), GL2(GL2alphabetagamma) and Gq(Gqalphabetagamma) are solubilized and purified | Bos taurus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.722 | - |
recombinant GL2 | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | activates phosphoinositide-specific phospholipase C in the presence of aluminium fluoride | Bos taurus | GDP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | alpha,beta,gamma, 1 * 42000 + 1 * 36000 (beta1) + 1 * 8000 (gamma1), GL1 or Gq protein, SDS-PAGE | Bos taurus |
? | alpha,beta,gamma, 1 * 43000 + 1 * 36000, (beta1) + 1 * 8000 (gamma2), GL1 protein, SDS-PAGE | Bos taurus |
? | alpha,beta,gamma, 1 * 39000 + 1 * 36000 (beta1) + 1 * 80000 (gamma2), Go protein, SDS-PAGE | Bos taurus |