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Literature summary for 3.6.4.B8 extracted from

  • Pan, M.; Santangelo, T.J.; Cubonova, L.; Li, Z.; Metangmo, H.; Ladner, J.; Hurwitz, J.; Reeve, J.N.; Kelman, Z.
    Thermococcus kodakarensis has two functional PCNA homologs but only one is required for viability (2013), Extremophiles, 17, 453-461.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.4.B8

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37200
-
 1 * 58100, subunit RFC-L, + 4 * 37200, subunit RFC-S, SDS-PAGE Thermococcus kodakarensis
58100
-
 1 * 58100, subunit RFC-L, + 4 * 37200, subunit RFC-S, SDS-PAGE Thermococcus kodakarensis
203000
-
 light scattering Thermococcus kodakarensis
208000
-
 gel filtration Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Thermococcus kodakarensis
-
 ADP + phosphate
-
 ?
ATP + H2O Thermococcus kodakarensis KW128
-
 ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis
-
-
-
Thermococcus kodakarensis KW128
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Thermococcus kodakarensis ADP + phosphate
-
 ?
ATP + H2O
-
 Thermococcus kodakarensis KW128 ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
pentamer 1 * 58100, subunit RFC-L, + 4 * 37200, subunit RFC-S, SDS-PAGE Thermococcus kodakarensis

Synonyms

Synonyms Comment Organism
replication factor C
-
 Thermococcus kodakarensis
RFC
-
 Thermococcus kodakarensis
RFC complex
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Thermococcus kodakarensis

General Information

General Information Comment Organism
evolution most archaeal genomes also encode two RFC homologs, designated the RFC large (RFC-L) and small (RFC-S) subunits, that assemble to form a pentameric complex that contains one RFC-L and four RFC-S subunits Thermococcus kodakarensis
physiological function proliferating cell nuclear antigen (PCNA) monomers assemble to form a ring-shaped clamp complex that encircles duplex DNA. PCNA binding to other proteins tethers them to the DNA providing contacts and interactions for many other enzymes essential for DNA metabolic processes. The PCNA clamp does not assemble autonomously but is loaded onto DNA by the replication factor C (RFC) clamp loader complex. RFC recognizes the 3' end of a single-strand/duplex DNA (primer-template) junction and uses the energy of ATP hydrolysis to assemble the PCNA ring around the primer. Primer extension by PolB is completely dependent on the presence of RFC plus either PCNA1 or PCNA2, and the rate of DNA synthesis increases when the PCNA1 or PCNA2 concentration is increased Thermococcus kodakarensis