Literature summary for 3.6.4.B8 extracted from

Seybert, A.; Singleton, M.R.; Cook, N.; Hall, D.R.; Wigley, D.B.
Communication between subunits within an archaeal clamp-loader complex (2006), EMBO J., 25, 2209-2218.

Search for more literature for 3.6.4.B8

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of replication factor C domains 1 and 2 at 2.1 A, crystal structure of replication factor C ADPNP complex at 3.5 A and at 4.0 A, hanging drop vapour diffusion method	Archaeoglobus fulgidus

Protein Variants

Protein Variants	Comment	Organism
additional information	mutation of the proposed arginine finger in the small subunits results in a complex that can still bind ATP but has impaired clamp-loading activity	Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	ATP	Km-values of mutant replication factor C complexes in the presence of DNA and Proliferating-Cell-Nuclear-Antigen (PCNA)	Archaeoglobus fulgidus
0.025	-	ATP	wild-type enzyme, in the presence of DNA and Proliferating-Cell-Nuclear-Antigen (PCNA), pH and temperature not specified in the publication	Archaeoglobus fulgidus

Organism

Organism	UniProt	Comment	Textmining
Archaeoglobus fulgidus	O28219 and O29072	O28219: Replication factor C small subunit, O29072: Replication factor C large subunit	-

Purification (Commentary)

Purification (Comment)	Organism
-	Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	-	Archaeoglobus fulgidus	ADP + phosphate	-	?

Subunits

Subunits	Comment	Organism
oligomer	communication between subunits in the RFC holoenzyme is investigated. The small subunit alone forms a hexameric ring that is six-fold symmetric in the absence of ATP. This symmetry is broken when the nucleotide is bound to the complex. The large conformational change observed may relate to the opening of PCNA rings that is required for them to be loaded onto DNA substrates	Archaeoglobus fulgidus

Synonyms

Synonyms	Comment	Organism
replication factor C	-	Archaeoglobus fulgidus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	ATP	turnover numbers of mutant replication factor C complexes in the presence of DNA and Proliferating-Cell-Nuclear-Antigen (PCNA)	Archaeoglobus fulgidus
1.7	-	ATP	wild-type enzyme, in the presence of DNA and Proliferating-Cell-Nuclear-Antigen (PCNA), pH and temperature not specified in the publication	Archaeoglobus fulgidus

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Release 2023.1 (January 2023)